Ultrastructural differences in presumed photoreceptive organs and molecular data as a means for species discrimination in Polygordius (Annelida, Protodriliformia, Polygordiidae)

Ultrastructural differences in presumed photoreceptive organs and molecular data as a means for species discrimination in Polygordius (Annelida, Protodriliformia, Polygordiidae)
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假定的感光器官和分子数据的超微结构差异作为Polygordius(环节动物、Protodriliformia、Polygordiidae)物种区分的手段

DOI:
10.1007/s13127-016-0272-8
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发表时间:
2016
影响因子:
1.6
通讯作者:
Purschke
Purschke
中科院分区:
生物学2区
文献类型:
--
作者:
Lehmacher;Ramey-Balci;Purschke

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Polygordiidae Czerniavsky,1881的成员分布于世界各地,是粗粒度海洋亚滨海沉积物的典型居民。在多毛类中,多毛科的种类是不寻常的,因为它们缺乏外部分节、副足、毛以及外部纤毛。形态特征的物种区分,从而限制了形状和微小结构的口前体和臀。因此,形态鉴定和描述的Polygordius种是困难的。在本研究中,我们使用分子和形态学方法提供了新的物种区分数据,即,DNA测序(COIgene)、透射电子显微镜(TEM)和共聚焦激光扫描显微镜(cLSM)。具体来说,我们调查是否光感受器样感觉器官,先前描述为Polygordius apapericulatusFraipont,1887年,可以提供额外的特征的分类价值,通过检查这些器官的超微结构在另外两个物种,Polygordius erythrophthalmusGiard,1880年和Polygordius lacteusSchneider,1868年。这些器官被发现出现在相对较大的数量,并包括不同类型的感觉细胞,包括一个横纹肌和两个纤毛,这是不同的组合,以形成小的感觉器官。在感觉器官的结构上,P.红眼吸虫P. lacteus;然而,当与P.无柄的这些结果与我们的分子数据表明P.红眼吸虫P. lacteus代表同一物种。ThusP. erythrophthalmus是一个无效种,代表P. lacteus。此外,这项研究表明,超微结构数据可以显着有助于物种的区分,特别是在形态相似的物种。
Members of Polygordiidae Czerniavsky, 1881 are distributed worldwide and are typical inhabitants of coarse grained, marine sublittoral sediments. Species in Polygordiidae are unusual among polychaetes because they lack external segmentation, parapodia, and chaetae as well as external ciliation. Morphological characters for species distinction are thus limited to the shape and minute structures of the prostomium and pygidium. As a result, morphological identification and description of thePolygordiusspecies is difficult. In the present study, we provide new data for species distinction using molecular and morphological methods, i.e., DNA-sequencing (COIgene), transmission electron microscopy (TEM), and confocal laser scanning microscopy (cLSM). Specifically, we investigated whether the photoreceptor-like sense organs, previously described forPolygordius appendiculatusFraipont, 1887, could provide additional characters of taxonomic value by examining the ultrastructure of these organs in two additional species,Polygordius erythrophthalmusGiard, 1880 andPolygordius lacteusSchneider, 1868. These organs were found to occur in comparatively large numbers and comprise different types of sensory cells including one rhabdomeric and two ciliary, which are variously combined to form small sensory organs. Structural differences in sensory organs were negligible betweenP. erythrophthalmusandP. lacteus; however, major differences were observed when compared toP. appendiculatus. These results are in accordance with our molecular data which indicated thatP. erythrophthalmusandP. lacteusrepresent the same species. ThusP. erythrophthalmusis an invalid species and represents a junior synonym ofP. lacteus. Furthermore, this study shows that ultrastructural data can significantly contribute to species distinction, especially in morphologically similar species.
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