Definition and estimation of resolution in single-particle reconstructions.
Definition and estimation of resolution in single-particle reconstructions.
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DOI：
10.1016/j.str.2010.05.008
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发表时间：
2010-07-14
期刊：
影响因子：
--
通讯作者：
Frank J
中科院分区：
文献类型：
--
作者：
Liao HY;Frank J
在本文中，我们审查了在单粒子重建中建立分辨率的当前实践。然后，比较了两种过程的重建，但是，这两个过程可能会引入两个半集之间的统计依赖性，这导致了过于乐观的分辨率估计。仅数据集的“真实”分辨率的一半的属性可以估计数据大小和分辨率之间的功能关系。 -Fator和数据收集的几何形状。
In this paper, we review current practices for establishing the resolution in single-particle reconstructions. The classical Raleigh criterion for the resolution is not applicable in this case; and the resolution is commonly defined by a consistency test, whereby the data set is randomly split in half and the two resulting reconstructions are then compared. Such a procedure, however, may introduce statistical dependence between the two half-sets, which leads to a too optimistic resolution estimate. On the other hand, this overestimation is counteracted by the diminished statistical properties of a mere half of the data set. The “true” resolution of the whole data set can be estimated when the functional relationship between the data size and the resolution is known. We are able to estimate this functional by taking into account the B-factor and the geometry of data collection. Finally, the drawbacks of resolution estimation are entirely avoided by computing the correlation of neighboring voxels in the Fourier domain.
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影响因子：
5.6
作者：
Grigorieff, N
通讯作者：
Grigorieff, N
影响因子：
5.6
作者：
Orlova, EV;Dube, P;vanHeel, M
通讯作者：
vanHeel, M
DOI：
10.1002/jemt.1060090405
发表时间：
1988-08-01
期刊：
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE
影响因子：
--
作者：
RADERMACHER, M
通讯作者：
RADERMACHER, M
影响因子：
2
作者：
KESSEL, M;RADERMACHER, M;FRANK, J
通讯作者：
FRANK, J
影响因子：
6.1
作者：
HENDERSON, R
通讯作者：
HENDERSON, R