Developmental onset of enduring long-term potentiation in mouse hippocampus.
Developmental onset of enduring long-term potentiation in mouse hippocampus.
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DOI：
10.1002/hipo.23257
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发表时间：
2020-12
期刊：
影响因子：
3.5
通讯作者：
Harris KM
中科院分区：
文献类型：
--
作者：
Ostrovskaya OI;Cao G;Eroglu C;Harris KM
对长时程增强(LTP)的分析为了解学习和记忆的细胞机制提供了一个强大的窗口。先前的工作表明，迟发性LTP(L-LTP)在出生后12天(P12)突然出现在大鼠海马区CA1区放射层，持续时间为3小时。这里的目标是确定导致L-LTP的小鼠海马区突触可塑性的发育概况。选择了两个已知影响突触可塑性的小鼠品系和两个突变：C57BL/6J和Fmr1−/y为背景，129 SVE和Hevin−/−(Sparcl1−/−)为背景。像大鼠一样，所有小鼠的海马片在发育早期都表现出测试脉冲诱导的抑郁，随着发育成熟5周，这种抑郁逐渐消失。所有品系小鼠的短时程增强表达在P10-P35之间呈渐进式递增，持续≤1小时。在129只SVE小鼠中，L-LTP发病(25%的切片)发生在3周，可靠的L-LTP(>；50%切片)出现在4周，而Hevin−/−将这一过程提前了1周。在C57BL/6J小鼠中，L-LTP的发病时间明显较晚，超过3-4周，直到5周才达到可靠性。虽然部分Fmr1−/y小鼠在3周前出现了L-LTP，但可靠的L-LTP也是在5周后才出现。在每隔90分钟或180分钟进行多次刺激的情况下，任何一种小鼠的L-长时程增强发作都不提前。这些发现表明，STP和L-LTP的发病存在重要的物种差异，这两种疾病在相同年龄的大鼠中发生，但在小鼠中是顺序获得的。
Analysis of long-term potentiation (LTP) provides a powerful window into cellular mechanisms of learning and memory. Prior work shows late LTP (L-LTP), lasting >3 hr, occurs abruptly at postnatal day 12 (P12) in the stratum radiatum of rat hippocampal area CA1. The goal here was to determine the developmental profile of synaptic plasticity leading to L-LTP in the mouse hippocampus. Two mouse strains and two mutations known to affect synaptic plasticity were chosen: C57BL/6J and Fmr1−/y on the C57BL/6J background, and 129SVE and Hevin−/− (Sparcl1−/−) on the 129SVE background. Like rats, hippocampal slices from all of the mice showed test pulse-induced depression early during development that was gradually resolved with maturation by 5 weeks. All the mouse strains showed a gradual progression between P10-P35 in the expression of short-term potentiation (STP), lasting ≤1 hr. In the 129SVE mice, L-LTP onset (>25% of slices) occurred by 3 weeks, reliable L-LTP (>50% slices) was achieved by 4 weeks, and Hevin−/− advanced this profile by 1 week. In the C57BL/6J mice, L-LTP onset occurred significantly later, over 3–4 weeks, and reliability was not achieved until 5 weeks. Although some of the Fmr1−/y mice showed L-LTP before 3 weeks, reliable L-LTP also was not achieved until 5 weeks. L-LTP onset was not advanced in any of the mouse genotypes by multiple bouts of theta-burst stimulation at 90 or 180 min intervals. These findings show important species differences in the onset of STP and L-LTP, which occur at the same age in rats but are sequentially acquired in mice.
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