Conserved Epigenetic Mechanisms Could Play a Key Role in Regulation of Photosynthesis and Development-Related Genes during Needle Development of Pinus radiata.
Conserved Epigenetic Mechanisms Could Play a Key Role in Regulation of Photosynthesis and Development-Related Genes during Needle Development of Pinus radiata.
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保守的表观遗传机制可能在调节光合作用和发育相关的基因的过程中起关键作用。
DOI：
10.1371/journal.pone.0126405
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Cañal MJ
中科院分区：
文献类型：
--
作者：
Valledor L;Pascual J;Meijón M;Escandón M;Cañal MJ
针叶成熟是一个复杂的过程，涉及细胞生长、分化和组织重塑，以获得完全的生理能力。叶诱导机制是众所周知的，然而，那些潜在的收购生理能力仍然知之甚少，特别是在针叶树。我们研究了特定的表观遗传调控的基因定义器官功能（PrRBCS和PrRBCA）和能力和应激反应（PrCSDP2和PrSHMT4）在三个阶段的针发展和一个去分化的控制。通过亚硫酸氢盐测序和染色质免疫沉淀（ChIP）分析DNA甲基化和组蛋白的基因特异性变化。PrRBCA和PrRBCS的表达在针叶成熟过程中增加，并且与H3K9me3、H3K27me3的进行性丢失和AcH4的增加相关。PrSHMT4的成熟相关沉默与H3K9me3水平的增加相关，而PrCSDP2的抑制与AcH4、H3K27me3、H3K9me3和特异性DNA甲基化之间的相互作用相关。HAT和HDAC抑制剂的使用导致进一步确定组蛋白乙酰化在调节我们的靶基因中的作用。这些结果与高通量分析在拟南芥和胡杨的整合表明，特定的表观遗传机制，调节光合作用基因之间的分析物种是保守的。
Needle maturation is a complex process that involves cell growth, differentiation and tissue remodelling towards the acquisition of full physiological competence. Leaf induction mechanisms are well known; however, those underlying the acquisition of physiological competence are still poorly understood, especially in conifers. We studied the specific epigenetic regulation of genes defining organ function (PrRBCS and PrRBCA) and competence and stress response (PrCSDP2 and PrSHMT4) during three stages of needle development and one de-differentiated control. Gene-specific changes in DNA methylation and histone were analysed by bisulfite sequencing and chromatin immunoprecipitation (ChIP). The expression of PrRBCA and PrRBCS increased during needle maturation and was associated with the progressive loss of H3K9me3, H3K27me3 and the increase in AcH4. The maturation-related silencing of PrSHMT4 was correlated with increased H3K9me3 levels, and the repression of PrCSDP2, to the interplay between AcH4, H3K27me3, H3K9me3 and specific DNA methylation. The employ of HAT and HDAC inhibitors led to a further determination of the role of histone acetylation in the regulation of our target genes. The integration of these results with high-throughput analyses in Arabidopsis thaliana and Populus trichocarpa suggests that the specific epigenetic mechanisms that regulate photosynthetic genes are conserved between the analysed species.
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影响因子：
5.1
作者：
Bennett, Tom;Leyser, Ottoline
通讯作者：
Leyser, Ottoline
影响因子：
7.2
作者：
Danker, Tania;Dreesen, Bjorn;Peterhaensel, Christoph
通讯作者：
Peterhaensel, Christoph
影响因子：
--
作者：
Cui, Long;Miao, Jun;Cui, Liwang
通讯作者：
Cui, Liwang
DOI：
10.1126/science.1190614
发表时间：
2010-10-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Feng S;Jacobsen SE;Reik W
通讯作者：
Reik W
影响因子：
5.3
作者：
De-la-Pena, Clelia;Nic-Can, Geovanny;Robert-Diaz, Manuel L.
通讯作者：
Robert-Diaz, Manuel L.