Feature tracking microfluidic analysis reveals differential roles of viscosity and friction in sickle cell blood.
Feature tracking microfluidic analysis reveals differential roles of viscosity and friction in sickle cell blood.
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DOI：
10.1039/d1lc01133b
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发表时间：
2022-04-12
期刊：
影响因子：
6.1
通讯作者：
中科院分区：
文献类型：
--
作者：
血液系统疾病中血流流变学的表征对于理解疾病的病理生理学至关重要。现有的方法来测量血液流变学参数是有限的，在他们的生理相关性，并有一个新的工具，专注于微循环和提取性能在更精细的分辨率比整体流动阻力的需要。在这里，我们提出了一种方法，结合微流体系统和强大的对象跟踪计算技术与数学建模分离的红细胞流动曲线成一个散装组件和壁组件。我们使用这个框架来评估有效粘度和壁摩擦力对镰状细胞病（SCD）患者血液在一系列氧张力下的整体阻力的不同贡献。我们的研究结果表明，SCD患者的血液在所有生理氧分压下都表现出较高的摩擦阻力和粘性阻力。此外，随着氧张力降低，粘性阻力比摩擦阻力增加得更快，这可能会混淆仅提取流速或总流动阻力的分析。此外，我们评估了输血治疗对耐药组分的影响，揭示了患者血液特性的变异性，这可能会提高我们对此类治疗临床反应异质性的理解。总的来说，我们的系统提供了一种新的方法来分析患者特定的血液特性，并可应用于广泛的血液和血管疾病。我们提出了一种方法，结合微流体系统和对象跟踪计算技术，以评估有效粘度和壁摩擦的贡献，从镰状细胞病患者的血液中的整体阻力。
Characterization of blood flow rheology in hematological disorders is critical for understanding disease pathophysiology. Existing methods to measure blood rheological parameters are limited in their physiological relevance, and there is a need for new tools that focus on the microcirculation and extract properties at finer resolution than overall flow resistance. Herein, we present a method that combines microfluidic systems and powerful object-tracking computational technologies with mathematical modeling to separate the red blood cell flow profile into a bulk component and a wall component. We use this framework to evaluate differential contributions of effective viscosity and wall friction to the overall resistance in blood from patients with sickle cell disease (SCD) under a range of oxygen tensions. Our results demonstrate that blood from patients with SCD exhibits elevated frictional and viscous resistances at all physiologic oxygen tensions. Additionally, the viscous resistance increases more rapidly than the frictional resistance as oxygen tension decreases, which may confound analyses that extract only flow velocities or overall flow resistances. Furthermore, we evaluate the impact of transfusion treatments on the components of the resistance, revealing patient variability in blood properties that may improve our understanding of the heterogeneity of clinical responses to such treatments. Overall, our system provides a new method to analyze patient-specific blood properties and can be applied to a wide range of hematological and vascular disorders. We present a method that combines microfluidic systems and object-tracking computational technologies to evaluate the contributions of effective viscosity and wall friction to the overall resistance in blood from patients with sickle cell disease.
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影响因子：
2.9
作者：
Henkelman, Sandra;Dijkstra-Tiekstra, Margriet J.;van Oeveren, Willem
通讯作者：
van Oeveren, Willem
影响因子：
2.9
作者：
Detterich J;Alexy T;Rabai M;Wenby R;Dongelyan A;Coates T;Wood J;Meiselman H
通讯作者：
Meiselman H
影响因子：
3.4
作者：
Lu, Xinran;Wood, David K.;Higgins, John M.
通讯作者：
Higgins, John M.
影响因子：
2.4
作者：
Embury, SH
通讯作者：
Embury, SH
DOI：
10.1073/pnas.0707122105
发表时间：
2007-12-18
影响因子：
11.1
作者：
Higgins, J. M.;Eddington, D. T.;Mahadevan, L.
通讯作者：
Mahadevan, L.