Spatial regulation of clathrin-mediated endocytosis through position-dependent site maturation.
Spatial regulation of clathrin-mediated endocytosis through position-dependent site maturation.
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DOI：
10.1083/jcb.202002160
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发表时间：
2020-11-02
期刊：
影响因子：
--
通讯作者：
Drubin DG
中科院分区：
文献类型：
--
作者：
Pedersen RTA;Hassinger JE;Marchando P;Drubin DG
Pedersen等人研究了网格蛋白介导的内吞途径受调控的步骤。他们报告了极化细胞内吞位点成熟率的位置依赖性差异，并表明货物通过调节位点成熟而不是位点起始来控制内吞内化。在网格蛋白介导的内吞作用（CME）中，超过50种不同的蛋白质在质膜上组装，将其重塑为装载货物的囊泡。长期以来，人们一直认为，货物触发本地CME网站组装在酿酒酵母的基础上发现，皮质肌动蛋白补丁，集群附近的胞吐位点，是CME网站。定量成像数据报告这里导致一个根本不同的看法，其中CME的步骤进行调节，哪些步骤是确定性的。我们定量和空间地描述了CME途径的进展，并确定了一个对货物敏感的监管过渡点，该点控制着从CME起始阶段到内化阶段的进展。因此，网站的成熟，而不是网站的启动，占先前观察到的极化分布肌动蛋白补丁在这个有机体。虽然以前的研究表明，货物通过调节CME启动率或流产事件的频率来确保其自身的内化，但我们的数据却通过通路中的检查点确定成熟为货物敏感步骤。
Pedersen et al. investigate steps of the clathrin-mediated endocytosis pathway that are subject to regulation. They report position-dependent differences in endocytic site maturation rates in polarized cells and suggest that cargo controls endocytic internalization through tuning site maturation rather than site initiation. During clathrin-mediated endocytosis (CME), over 50 different proteins assemble on the plasma membrane to reshape it into a cargo-laden vesicle. It has long been assumed that cargo triggers local CME site assembly in Saccharomyces cerevisiae based on the discovery that cortical actin patches, which cluster near exocytic sites, are CME sites. Quantitative imaging data reported here lead to a radically different view of which CME steps are regulated and which steps are deterministic. We quantitatively and spatially describe progression through the CME pathway and pinpoint a cargo-sensitive regulatory transition point that governs progression from the initiation phase of CME to the internalization phase. Thus, site maturation, rather than site initiation, accounts for the previously observed polarized distribution of actin patches in this organism. While previous studies suggested that cargo ensures its own internalization by regulating either CME initiation rates or frequency of abortive events, our data instead identify maturation through a checkpoint in the pathway as the cargo-sensitive step.
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影响因子：
21.3
作者：
通讯作者：
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DOI：
10.1083/jcb.144.6.1203
发表时间：
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期刊：
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影响因子：
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