Oxidative Stress in the Dahl Salt-Sensitive Hypertensive Rat
Oxidative Stress in the Dahl Salt-Sensitive Hypertensive Rat
复制标题
达尔盐敏感性高血压大鼠的氧化应激
DOI：
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发表时间：
2005
影响因子：
12.3
通讯作者：
K. Kanmatsuse
中科院分区：
文献类型：
--
作者：
T. Kushiro;H. Fujita;R. Hisaki;T. Asai;Izumi Ichiyama;Yasuyuki Kitahara;M. Koike;H. Sugiura;F. Saito;Y. Otsuka;K. Kanmatsuse
氧化应激被认为在高血压的发病机制中起重要作用。测定8-iso前列腺素F2α(8-iso prostaglandin F2 DNA，8-iso prostaglandin F2)可用于体内氧化应激的评估。8-ISO是F2-异前列腺素的主要尿代谢物，由超氧阴离子自由基攻击花生四烯酸形成。我们检测了Dahl盐敏感(Dahl-S)大鼠和Dahl耐盐(Dahl-R)大鼠的氧化应激水平。采用高盐饮食(8%氯化钠；HS)和低盐饮食(0.3%氯化钠；LS)饲养Dahl-S和Dahl-R大鼠3周，测定血压和24小时尿8-异丙基(U-8-ISO)排泄量。在Dahl-S大鼠中，高盐饮食诱发高血压(LS组139±3 mm Hg，HS组186±2 mm Hg，p<；0.05)，并显著增加U-8-ISO(LS组24.9±3.6 ng/24 hr，HS组63.2±14.6 ng/24 h，p<；0.05)。在高盐和低盐处理的Dahl-R大鼠之间，血压和U-8-ISO没有显著差异。四个实验组的U-8-ISO浓度与收缩压均呈正相关(r=0.866)。此外，达尔-S-8%组大鼠尿8-羟基-2‘-脱氧鸟苷(U-8-OHdG)水平高于达尔-S-0.3%组(LS组为136.1±48.4 ng/24小时，HS组为322.8±46.7 ng/24小时，p<；0.05)，且U-8-OHdG与SBP呈正相关(r=0.681)。这些结果提示氧自由基参与了高血压的发病机制。
Oxidative stress has been proposed as important in the pathogenesis of hypertension. Measurement of 8-iso prostaglandin F2α(8-ISO) is introduced for evaluating oxidative stress in vivo. 8-ISO is the major urinary metabolite of F2-isoprostanes and is formed nonenzymatically from the attack of superoxide radicals on arachidonic acid. We examined the oxidative stress level in the Dahl salt-sensitive (Dahl-S) rats and the Dahl salt-resistant (Dahl-R) rats. Dahl-S and Dahl-R rats were fed either a high salt diet (8% NaCl; HS) or low salt diet (0.3% NaCl; LS) for 3 weeks, and systolic blood pressure (SBP) and 24-hr urinary excretion of 8-ISO (U-8-ISO) were measured. In Dahl-S rats, the high salt diet induced hypertension (139 ± 3 mmHg in LS versus 186 ± 2 mmHg in HS, p < .05) and significantly increased the U-8-ISO (24.9 ± 3.6 ng/24 hr in LS versus 63.2 ± 14.6 ng/24 hr in HS, p < .05). No significant difference in blood pressure or U-8-ISO was observed between high-salt and low-salt treated Dahl-R rats. U-8-ISO concentration was correlated with SBP in all four experimental groups (r = 0.866). Moreover, urinary 8-hydroxy-2′-deoxyguanosine (U-8-OHdG), which is one of the most commonly used markers for evaluation of oxidative stress, was higher in Dahl-S-8% rats than in Dahl-S-0.3% rats (136.1 ± 48.4 ng/24 hr in LS versus 322.8 ± 46.7 ng/24 hr in HS, p < .05), and U-8-OHdG was correlated with SBP (r = 0.681) in Dahl-S rats. These results suggest oxygen radicals are involved in the pathogenesis of hypertension.
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影响因子：
20.1
作者：
Katherine A. Shen;F. DeLano;Benjamin W. Zweifach;G. Schmid-Schönbein
通讯作者：
Katherine A. Shen;F. DeLano;Benjamin W. Zweifach;G. Schmid-Schönbein
一氧化氮、超氧化物和过氧亚硝酸盐：好的、坏的和丑陋的。
DOI：
10.1152/ajpcell.1996.271.5.c1424
发表时间：
1996
期刊：
The American journal of physiology
影响因子：
--
作者：
Beckman,JS;Koppenol,WH
通讯作者：
Koppenol,WH
DOI：
10.1073/pnas.88.11.4680
发表时间：
1991-06-01
影响因子：
11.1
作者：
GELVAN, D;SALTMAN, P;POWELL, SR
通讯作者：
POWELL, SR
DOI：
10.1073/pnas.86.24.9697
发表时间：
1989-12-01
影响因子：
11.1
作者：
SHIGENAGA, MK;GIMENO, CJ;AMES, BN
通讯作者：
AMES, BN
影响因子：
8.3
作者：
Swei, A;Lacy, F;Schmid-Schönbein, GW
通讯作者：
Schmid-Schönbein, GW