Topological defects of integer charge in cell monolayers.
Topological defects of integer charge in cell monolayers.
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DOI：
10.1039/d1sm00100k
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发表时间：
2021-06-28
期刊：
影响因子：
3.4
通讯作者：
Serra F
中科院分区：
文献类型：
--
作者：
Endresen KD;Kim M;Pittman M;Chen Y;Serra F
许多细胞类型自发地像液晶一样有序，因此它们形成拓扑缺陷，这影响了细胞组织。虽然拓扑电荷为±1/2的缺陷在细胞单层中很常见，但电荷为±1的缺陷被认为与生命系统中突起的形成有关，更难以捉摸。我们使用拓扑图案在细胞单层的受控位置施加±1的拓扑电荷。我们研究了两种类型的细胞，3 T6成纤维细胞和EpH-4上皮细胞，我们比较了它们在这种模式下的行为，表征了对齐程度，缺陷附近的细胞密度，以及它们在缺陷核心的行为。我们观察到密度变化的3 T6单层附近的两种类型的缺陷在相同的长度尺度。通过选择适当的几何参数，我们的形貌特征，我们确定了一个新的行为的3 T6细胞附近的缺陷与拓扑电荷+1，导致细胞的首选形状的变化。我们的策略允许一个很好的控制单元排列附近的缺陷作为一个平台，研究液晶性能的细胞。使用微尺度的地形图案，引导成纤维细胞和上皮细胞形成单层的拓扑缺陷，我们检查依赖于细胞类型以及拓扑电荷的行为。
Many cell types spontaneously order like nematic liquid crystals, and, as such, they form topological defects, which influence the cell organization. While defects with topological charge ±1/2 are common in cell monolayers, defects with charge ±1, which are thought to be relevant in the formation of protrusions in living systems, are more elusive. We use topographical patterns to impose topological charge of ±1 in controlled locations in cell monolayers. We study two types of cells, 3T6 fibroblasts and EpH-4 epithelial cells, and we compare their behavior on such patterns, characterizing the degree of alignment, the cell density near the defects, and their behavior at the defect core. We observe density variation in the 3T6 monolayers near both types of defects over the same length-scale. By choosing appropriate geometrical parameters of our topographical features, we identify a new behavior of 3T6 cells near the defects with topological charge +1, leading to a change in the cells’ preferred shape. Our strategy allows a fine control of cell alignment near defects as a platform to study liquid crystalline properties of cells. Using microscale topographic patterns to guide fibroblasts and epithelial cells to form topological defects in monolayers, we examine behaviors dependent on cell type as well as topological charge.
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影响因子：
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