Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus.
Morphological and molecular characterization of Cystoisospora sp. from Asian small-clawed otters Aonyx cinereus.
复制标题
囊肿的形态和分子表征。来自亚洲小爪的Otters aonyx Cinereus。
DOI：
10.1016/j.ijppaw.2018.07.001
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Ike K
中科院分区：
文献类型：
--
作者：
Tokiwa T;Ohnuki A;Kubota R;Tamukai K;Ike K
在这项研究中，我们提供了亚洲小爪水獭（Aonyx cinereus）的囊等孢子虫感染的第一个描述。2017年7月，最近从印度尼西亚共和国进口的三只幼年水獭出现严重腹泻，并被诊断为球虫感染;其中两只最终死亡。粪便检查发现存在大量卵囊。孢子化卵囊具有典型的囊等孢子虫特征，大小为24.6 ± 1.6（22.0-27.0）× 21.8 ± 1.4（19.0-25.0）μm，卵囊长宽比为1.1 ± 0.1（1.0-1.3）。每个孢子囊含有四个子孢子，呈首尾相连的排列。无Stieda小体，存在孢子囊残留。这些形态学特征将本种与其他有效的从鼬科动物中描述的Cystoisospora种区分开来。在两个位点进行分子分析：核18 S核糖体RNA和线粒体细胞色素c氧化酶亚基I基因。18 S序列与犬Cystoispora ohioensis具有高度相似性（1-bp差异，1422/1423 [99.9%]）。在细胞色素c氧化酶亚基I基因位点，水獭的序列与猫Cystoispora rivolta的序列相同（847/847 [100%]）。系统发育分析表明，水獭和C. rivolta聚在同一个Cystoisospora分支中。基于这些数据，我们得出结论，从水獭中检测到的Cystoisospora sp.似乎与C. rivolta。我们的特点是囊等孢子虫感染在三个亚洲小爪水獭。两只水獭出现厌食、绿色腹泻和呕吐。粪便检查发现大量卵囊具有典型的囊等孢子虫特征。孢子化卵囊在形态上与其他囊等孢菌属的卵囊不同。从鼬科动物身上分子和形态特征与猫C高度相似。rivolta。
In this study, we provide the first description of Cystoisospora infection in Asian small-clawed otters (Aonyx cinereus). In July 2017, three juvenile otters recently imported from the Republic of Indonesia showed severe diarrhea and were diagnosed with coccidial infection; two of them eventually died. Fecal examination revealed the presence of numerous oocysts. Sporulated oocysts showed typical Cystoisospora features, measuring 24.6 ± 1.6 (22.0–27.0) × 21.8 ± 1.4 (19.0–25.0) μm, with an oocyst length/width ratio of 1.1 ± 0.1 (1.0–1.3). Each sporocyst contained four sporozoites in a head-to-tail arrangement. The Stieda body was absent, and the sporocyst residuum was present. These morphological characteristics differentiated this species from the other valid Cystoisospora species described from mustelids. Molecular analysis was conducted at two loci: the nuclear 18S ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes. The 18S sequence showed high similarity with canine Cystoisispora ohioensis (1-bp difference, 1422/1423 [99.9%]). At the cytochrome c oxidase subunit I gene locus, the sequence from otters was identical to that of feline Cystoisospora rivolta (847/847 [100%]). Phylogenetic analyses using concatenated data demonstrated that Cystoisospora sp. from otters and C. rivolta grouped together in the same Cystoisospora clade. Based on these data, we concluded that Cystoisospora sp. detected from otters appeared to be highly similar to C. rivolta. We characterized Cystoisospora infection in three Asian small-clawed otters. Two otters showed anorexia, green diarrhea, and vomiting. Fecal examination revealed numerous oocysts with typical Cystoisospora features. Sporulated oocysts were morphologically differentiated from those of other Cystoisospora spp. from mustelids. Molecular and morphological features were highly similar to feline C. rivolta.
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