Overexpression of p53 and long-term survival in colon carcinoma.
Overexpression of p53 and long-term survival in colon carcinoma.
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DOI：
10.1038/bjc.1994.295
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发表时间：
1994-08
影响因子：
8.8
通讯作者：
Hakama, M.
中科院分区：
文献类型：
--
作者：
Auvinen, A.;Isola, J.;Visakorpi, T.;Koivula, T.;Virtanen, S.;Hakama, M.
对坦佩雷大学医院1971 ~ 1982年12年间确诊的144例经组织学证实的结肠癌病例进行生存分析，以检验p53过表达与不良临床结局相关的假设。石蜡包埋的切片，使用多克隆抗体CM-1对p53蛋白进行免疫组织化学染色，以确定异常表达的p53肿瘤抑制基因。69%的肿瘤（100/144）显示p53蛋白过度表达。p53过度表达的患病率与患者的年龄和性别以及肿瘤的亚部位无关，但在晚期肿瘤中略高于局部肿瘤，尽管无统计学显著性。过表达与较高的S期分数相关。还观察到在过度表达的肿瘤中，非整倍体肿瘤的比例更大的一些迹象，尽管这一发现没有达到统计学意义。p53过表达肿瘤患者的5年生存率为37%，而p53正常表达肿瘤患者的5年生存率为58%。相应的10年发生率为34%和54%。在使用考克斯模型的多变量分析中，生存差异与患者的年龄和性别以及肿瘤的亚部位和分期无关。此外，在一个肿瘤子集的分析中，在控制流式细胞术参数后，p53过表达的影响仍然存在。因此，p53过表达似乎是一个有用的结肠癌预后指标。
Survival analysis of 144 histologically confirmed cases of colon carcinoma diagnosed in a 12 year period (1971-82) at the Tampere University Hospital was performed to test the hypothesis that p53 overexpression is associated with a poor clinical outcome. Immunohistochemical staining of paraffin-embedded sections using a polyclonal antibody CM-1 against p53 protein was performed to identify aberrant expression of the p53 tumour-suppressor gene. Sixty-nine per cent of the tumours (100/144) showed overexpression of the p53 protein. The prevalence of p53 overexpression was independent of age and sex of the patient and subsite of the tumour, but was slightly, although not statistically significantly, higher in advanced than in localised tumours. Overexpression was associated with a higher S-phase fraction. Some indication of a larger proportion of aneuploid tumours among those with overexpression was also observed, although this finding did not reach statistical significance. Significantly reduced patient survival for tumours with p53 overexpression was found. Patients with p53-overexpressing tumours had a corrected 5 year survival rate of 37% compared with 58% among patients whose tumours had normal expression of p53. The corresponding 10 year rates were 34% and 54%. In the multivariate analysis using a Cox model, the survival difference was independent of age and sex of the patient, as well as of subsite and stage of the tumour. Furthermore, the effect of p53 overexpression remained after controlling for flow cytometric parameters in an analysis of a subset of tumours. Thus, p53 overexpression appears to be a useful prognostic indicator in colon carcinoma.
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影响因子：
64.5
作者：
ELDEIRY, WS;TOKINO, T;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
DOI：
10.1073/pnas.87.19.7555
发表时间：
1990-10-01
影响因子：
11.1
作者：
RODRIGUES, NR;ROWAN, A;LANE, DP
通讯作者：
LANE, DP
影响因子：
168.9
作者：
SUN, XF;CARSTENSEN, JM;NORDENSKJOLD, B
通讯作者：
NORDENSKJOLD, B
DOI：
10.1093/jnci/84.11.883
发表时间：
1992-06-03
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
VISAKORPI, T;KALLIONIEMI, OP;ISOLA, J
通讯作者：
ISOLA, J
影响因子：
7.3
作者：
VANDENBERG, FM;TIGGES, AJ;WALBOOMERS, JMM
通讯作者：
WALBOOMERS, JMM