Polarized release of enveloped viruses in the embryonic chick heart: demonstration of epithelial polarity in the presumptive myocardium.

Polarized release of enveloped viruses in the embryonic chick heart: demonstration of epithelial polarity in the presumptive myocardium.
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胚胎鸡心脏中包膜病毒的极化释放:假定心肌中上皮极性的证明。

DOI:
10.1016/0012-1606(90)90111-u
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发表时间:
1990
影响因子:
2.7
通讯作者:
Fischman,DA
Fischman,DA
中科院分区:
生物学3区
文献类型:
--
作者:
Peng,I;Dennis,JE;Rodriguez-Boulan,E;Fischman,DA

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脊椎动物胚胎心脏的假定心肌是由立方上皮细胞组成的。为了检查这些发育中的肌细胞的功能极性,用流感病毒(FLU)或水疱性口炎病毒(VSV)感染鸡胚心脏(Hamburger-Hamilton阶段10-13)。这些病毒已被证明在极化肾(MDCK)细胞的单层中以矢量方式分选到顶端(FLU)或基底外侧(VSV)膜表面。我们的研究结果表明,这些病毒芽具有可比的极性从分化的肌细胞。然而,这两种病毒的极化出芽似乎存在阶段依赖性差异:VSV的限制性基底外侧释放存在于心管形成之前或之后不久,而FLU的极化出芽则在发育后期建立。这些结果进行了讨论,在心脏发育的早期阶段的质膜组织。
The presumptive myocardium of the embryonic vertebrate heart is composed of cells which exhibit the morphology of a cuboidal epithelium. To examine the functional polarity of these developing myocytes, embryonic chick hearts (Hamburger-Hamilton stages 10–13) were infected with either influenza virus (FLU) or vesicular stomatitis virus (VSV). These viruses have been shown to sort vectorially to either apical (FLU) or basolateral (VSV) membrane surfaces in monolayers of polarized kidney (MDCK) cells. Our results demonstrate that these viruses bud with comparable polarity from differentiating myocytes. However, there appear to be stage-dependent differences in the polarized budding of the two viruses: restricted basolateral release of VSV is present before or shortly after the formation of the heart tube, whereas polarized budding of FLU is established later in development. These results are discussed in terms of plasma membrane organization during the early stages of cardiac development.
DOI: 10.1126/science.2672330
发表时间: 1989-08-18
期刊: SCIENCE
影响因子: 56.9
作者:
RODRIGUEZBOULAN, E;NELSON, WJ
通讯作者: NELSON, WJ
DOI: 10.1159/000145781
发表时间: 1983
期刊: Acta anatomica
影响因子: --
作者:
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通讯作者: J. Hurlé
DOI: 10.1083/jcb.96.3.866
发表时间: 1983-03
影响因子: 7.8
作者:
Rodriguez-Boulan, E;Paskiet, K T;Sabatini, D D
通讯作者: Sabatini, D D
DOI: 10.1083/jcb.104.4.905
发表时间: 1987-04
期刊: The Journal of cell biology
影响因子: --
作者:
Vega-Salas DE;Salas PJ;Gundersen D;Rodriguez-Boulan E
通讯作者: Rodriguez-Boulan E
肌原纤维发生和 Z 带分化
DOI: --
发表时间: 1969
期刊: The Anatomical Record
影响因子: --
作者:
D. E. Kelly
通讯作者: D. E. Kelly