Development of a method to extract and purify target compounds from medicinal plants in a single step: online hyphenation of expanded bed adsorption chromatography and countercurrent chromatography.
Development of a method to extract and purify target compounds from medicinal plants in a single step: online hyphenation of expanded bed adsorption chromatography and countercurrent chromatography.
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开发一种从单一步骤中从药用植物中提取和净化靶化合物的方法：扩展的床吸附色谱和反流色谱法的在线连字符。
DOI：
10.1021/ac4035955
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发表时间：
2014-04-01
影响因子：
7.4
通讯作者：
Hu, Ping
中科院分区：
文献类型：
--
作者：
Li, Yang;Wang, Nan;Zhang, Min;Ito, Yoichiro;Zhang, Hongyang;Wang, Yuerong;Guo, Xin;Hu, Ping
从天然资源中提取和纯化的纯化合物对于先导化合物发现和药物筛选至关重要。本研究提出了一种新的二维膨胀床吸附色谱（EBAC）-高速逆流色谱（HSCCC）联用技术，用于药用植物中目标化合物的一步提取和纯化。EBAC和HSCCC通过六端口喷射阀作为接口连接。在该系统上对丹参和黄连的有效成分进行了分离，以验证其功效。通过二维系统在一个步骤中从丹参中获得52.9 mg的丹参素酸B和2.1 mg的迷迭香酸。目标产物丹参素B和迷迭香酸的纯度分别达到96%和74%以上。从黄连中分离得到黄连碱4.6mg，小檗碱4.1mg，纯度分别为98%和82%。处理时间是多步法的近50%。这些结果表明，本方法是一种快速和绿色的方式收获目标从药用植物在一个单一的步骤。
Pure compounds extracted and purified from natural sources are crucial to lead discovery and drug screening. This study presents a novel two-dimensional hyphenation of expanded bed adsorption chromatography (EBAC) and high-speed countercurrent chromatography (HSCCC) for extraction and purification of target compounds from medicinal plants in a single step. The EBAC and HSCCC were hyphenated via a six-port injection valve as an interface. Fractionation of ingredients of Salvia miltiorrhiza and Rhizoma coptidis was performed on the hyphenated system to verify its efficacy. An amount each of 52.9 mg of salvianolic acid B and 2.1 mg of rosmarinic acid was obtained from Salvia miltiorrhiza by the two-dimensional system in a single step. The purities of the targets were over 96% of salvianolic acid B and 74% of rosmarinic acid. An amount each of 4.6 mg of coptisine and 4.1 mg of berberine was obtained from Rhizoma coptidis each with 98% and 82% purity, respectively. The processing time was nearly 50% that of the multi-step method. These results indicate that the present method is a rapid and green way to harvest targets from medicinal plants in a single step.
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影响因子：
7.4
作者：
Franco, P;Blanc, J;Minguillón, C
通讯作者：
Minguillón, C
二维逆流色谱：第一传统逆流色谱、第二酸碱洗脱逆流色谱
影响因子：
4.1
作者：
Hu, Ruilin;Dai, Xiaojing;Pan, Yuanjiang
通讯作者：
Pan, Yuanjiang
影响因子：
4.1
作者：
Zhang, Min;Ignatova, Svetlana;Sutherland, Ian
通讯作者：
Sutherland, Ian
集成膨胀床吸附原位提取分离丹参中丹酚酸B
影响因子：
8.6
作者：
Zhang, Min;Yang, Huihua;Hu, Ping
通讯作者：
Hu, Ping
影响因子：
4.1
作者：
Weisz, Adrian;Witten, Jacob J.;Ito, Yoichiro
通讯作者：
Ito, Yoichiro