Caged neuron MEA: a system for long-term investigation of cultured neural network connectivity.
Caged neuron MEA: a system for long-term investigation of cultured neural network connectivity.
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DOI：
10.1016/j.jneumeth.2008.07.023
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发表时间：
2008-10-30
影响因子：
3
通讯作者：
Pine, Jerome
中科院分区：
文献类型：
--
作者：
Erickson, Jonathan;Tooker, Angela;Tai, Y. -C.;Pine, Jerome
用于研究培养神经网络的传统技术（例如膜片钳和多电极阵列）受到以下限制：1）可以同时电接触的已识别细胞的数量，2）可以研究细胞的时间长度，以及3）缺乏一对一的神经元到电极的特异性。在这里，我们提出了一种新设备——笼式神经元多电极阵列——它克服了这些限制。这种微机械设备由一系列神经笼组成，这些神经笼以机械方式将神经元捕获在细胞外电极附近。当细胞体被困住时，轴突和树突可以自由生长到周围区域，形成网络。电极是双向的，能够刺激和记录动作电位。该系统是非侵入性的，因此可以通过稳定的一对一神经元与电极对应关系来研究网络的所有组成神经元的整个生命周期。概念验证实验旨在说明功能网络在由 4×4 阵列中的 16 个笼子组成的神经芯片系统中形成，并且可以在几周内完全绘制阈上连接。神经芯片为研究小型培养神经网络开辟了神经生物学的新领域。
Traditional techniques for investigating cultured neural networks, such as the patch clamp and multi-electrode array, are limited by: 1) the number of identified cells which can be simultaneously electrically contacted, 2) the length of time for which cells can be studied, and 3) the lack of one-to-one neuron-to-electrode specificity. Here, we present a new device—the caged neuron multi-electrode array—which overcomes these limitations. This micro-machined device consists of an array of neurocages which mechanically trap a neuron near an extracellular electrode. While the cell body is trapped, the axon and dendrites can freely grow into the surrounding area to form a network. The electrode is bi-directional, capable of both stimulating and recording action potentials. This system is non-invasive, so that all constituent neurons of a network can be studied over its lifetime with stable one-to-one neuron-to-electrode correspondence. Proof-of-concept experiments are described to illustrate that functional networks form in a neurochip system of 16 cages in a 4×4 array, and that suprathreshold connectivity can be fully mapped over several weeks. The neurochip opens a new domain in neurobiology for studying small cultured neural networks.
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影响因子：
64.8
作者：
Bi, GQ;Poo, MM
通讯作者：
Poo, MM
影响因子：
3.4
作者：
CHIEN, CB;PINE, J
通讯作者：
PINE, J
影响因子：
3
作者：
CHIEN, CB;PINE, J
通讯作者：
PINE, J
影响因子：
1.2
作者：
Holt, GR;Koch, C
通讯作者：
Koch, C
影响因子：
64.8
作者：
Fitzsimonds, RM;Song, HJ;Poo, MM
通讯作者：
Poo, MM