miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice.
miR2118 Negatively Regulates Bacterial Blight Resistance through Targeting Several Disease Resistance Genes in Rice.
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DOI：
10.3390/plants12223815
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发表时间：
2023-11-09
期刊：
影响因子：
--
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Zhu X;Kuang Y;Chen Y;Shi J;Cao Y;Hu J;Yu C;Yang F;Tian F;Chen H
植物miRNAs是一类长度为21-24nt的非编码RNA，在植物对生物和非生物胁迫的响应中发挥着重要作用。由水稻黄单胞菌(Xanthomonas Oryzae)引起的白叶枯病。水稻白叶枯病是水稻上最严重的细菌性病害之一。我们以前的工作表明OSA-miR2118b/n是由Xoo感染诱导的。然而，miR2118的生物学功能尚未在实验中得到表征。在此，我们利用CRISPR/Cas9构建了MIR2118b OE，以及MIR2118b/n的单、双突变体。进一步的结果表明，接种Xoo后，OSA-MIR2118b OE植株比野生型植株的病斑长度长，而MIR2118 CRISPR植株的病斑长度比野生型植株短。在水稻原生质体中的共转化实验表明，OSA-miR2118对miR2118的3个核苷酸结合位点和富亮氨酸重复序列基因(LOC_Os08g42700.1、LOC_Os01g05600.1和LOC_Os12g37290.1)的转录产物进行了负调控，但不能调控在结合位点中心插入3个碱基的突变的NLR基因。在MIR2118b OE和MIR2118b CRISPR植株中，这三个NLR基因的转录水平与OSA-miR2118相反。以上结果表明，OSA-miR2118b/n通过负调控多个NLR基因对白叶枯病的抗性进行负调控。
Plant miRNAs are a class of noncoding RNA with a length of 21–24 nt that play an important role in plant responses to biotic and abiotic stresses. Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases in rice. Our previous work showed that osa-miR2118b/n was induced by Xoo infection. However, the biological function of miR2118 has not yet been characterized in experiments. Herein, we constructed MIR2118b OE, as well as single and double mutants of MIR2118b/n using CRISPR/Cas9. Further results showed that osa-MIR2118b OE plants exhibited longer lesion lengths than the wild type after Xoo inoculation, while MIR2118 CRISPR plants exhibited shorter lesion lengths than the wild type after Xoo inoculation. Co-transformation experiments in rice protoplasts indicated that osa-miR2118 negatively regulated the transcripts of three nucleotide-binding sites and leucine-rich repeat (NLR) genes (LOC_Os08g42700.1, LOC_Os01g05600.1, and LOC_Os12g37290.1) which are predicted target genes of miR2118, but not the mutated NLR genes with a 3 bp insertion at the center of the binding sites. The transcriptional level of the three NLR genes was reversed relative to osa-miR2118 in the MIR2118b OE and MIR2118b CRISPR plants. The above results demonstrate that osa-miR2118b/n negatively regulates the resistance to bacterial blight through negatively regulating several NLR genes.
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水稻先天免疫中的microRNA。
DOI：
10.1186/s12284-016-0078-5
发表时间：
2016-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Baldrich P;San Segundo B
通讯作者：
San Segundo B
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
3.3
作者：
de Vries S;Kloesges T;Rose LE
通讯作者：
Rose LE
影响因子：
9.2
作者：
Addo-Quaye, Charles;Eshoo, Tifani W.;Axtell, Michael J.
通讯作者：
Axtell, Michael J.
影响因子：
4.9
作者：
Chen, Songbiao;Tao, Lizen;Wang, Guo-Liang
通讯作者：
Wang, Guo-Liang