The DSL ligand APX-1 is required for normal ovulation in C. elegans.
The DSL ligand APX-1 is required for normal ovulation in C. elegans.
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DOI：
10.1016/j.ydbio.2018.01.009
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发表时间：
2018-03-15
影响因子：
2.7
通讯作者：
Hubbard EJA
中科院分区：
文献类型：
--
作者：
McGovern M;Castaneda PG;Pekar O;Vallier LG;Cram EJ;Hubbard EJA
DSL配体在后生动物的许多细胞环境中激活Notch受体以指定细胞命运。此外，Notch受体活性与有丝分裂后形态发生和神经元功能有关。In C.在线虫中，DSL家族配体APX-1在近端性腺谱系的细胞亚群中表达，在那里它可以充当潜在的增殖促进信号以维持近端生殖系肿瘤。在这里，我们检测了近端性腺中的apx-1，并揭示了其在维持正常排卵中的作用。apx-1的缺失导致内有丝分裂卵母细胞（Emo）表型和排卵缺陷。我们发现lag-2可以替代apx-1发挥这一作用，排卵缺陷会因ipp-5的缺失而部分受到抑制，而lin-12的缺失会导致类似的表型。此外，我们发现排卵缺陷往往伴随着卵母细胞进入后受精囊远端颈部关闭延迟。虽然受精囊内发生钙振荡，但当远端颈部未完全关闭时，钙信号异常。此外，卵母细胞有时不能正确地通过受精囊，一旦颈部关闭，导致卵母细胞破碎。最后，异常卵母细胞和颈部闭合缺陷时，偶尔看到apx-1或lin-12活性降低成年动物，这表明可能的发育后APX-1和LIN-12信号在排卵中的作用。
DSL ligands activate the Notch receptor in many cellular contexts across metazoa to specify cell fate. In addition, Notch receptor activity is implicated in post-mitotic morphogenesis and neuronal function. In C. elegans, the DSL family ligand APX-1 is expressed in a subset of cells of the proximal gonad lineage, where it can act as a latent proliferation-promoting signal to maintain proximal germline tumors. Here we examine apx-1 in the proximal gonad and uncover a role in the maintenance of normal ovulation. Depletion of apx-1 causes an endomitotic oocyte (Emo) phenotype and ovulation defects. We find that lag-2 can substitute for apx-1 in this role, that the ovulation defect is partially suppressed by loss of ipp-5, and that lin-12 depletion causes a similar phenotype. In addition, we find that the ovulation defects are often accompanied by a delay of spermathecal distal neck closure after oocyte entry. Although calcium oscillations occur in the spermatheca, calcium signals are abnormal when the distal neck does not close completely. Moreover, oocytes sometimes cannot properly transit through the spermatheca, leading to fragmentation of oocytes once the neck closes. Finally, abnormal oocytes and neck closure defects are seen occasionally when apx-1 or lin-12 activity is reduced in adult animals, suggesting a possible post-developmental role for APX-1 and LIN-12 signaling in ovulation.
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影响因子：
4.5
作者：
Kovacevic I;Orozco JM;Cram EJ
通讯作者：
Cram EJ
影响因子：
2.4
作者：
Chao MY;Larkins-Ford J;Tucey TM;Hart AC
通讯作者：
Hart AC
DOI：
10.1083/jcb.134.3.699
发表时间：
1996-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Iwasaki K;McCarter J;Francis R;Schedl T
通讯作者：
Schedl T
影响因子：
7.7
作者：
Li P;Collins KM;Koelle MR;Shen K
通讯作者：
Shen K
影响因子：
56.9
作者：
Miller, MA;Nguyen, VQ;Greenstein, D
通讯作者：
Greenstein, D