LncRNA TUG1 attenuates ischaemia-reperfusion-induced apoptosis of renal tubular epithelial cells by sponging miR-144-3p via targeting Nrf2.
LncRNA TUG1 attenuates ischaemia-reperfusion-induced apoptosis of renal tubular epithelial cells by sponging miR-144-3p via targeting Nrf2.
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LncRNA TUG1 通过靶向 Nrf2 海绵 miR-144-3p 减弱缺血再灌注诱导的肾小管上皮细胞凋亡
DOI：
10.1111/jcmm.16924
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发表时间：
2021-10
影响因子：
5.3
通讯作者：
Cheng F
中科院分区：
文献类型：
--
作者：
Zhao S;Chen W;Li W;Yu W;Li S;Rao T;Ruan Y;Zhou X;Liu C;Qi Y;Cheng F
肾缺血/再灌注（I/R）损伤可导致肾脏损伤和功能障碍，氧化应激和细胞凋亡在其中起重要作用。长链非编码RNA（lncRNA）和微小RNA（miRNAs）与肾I/R密切相关，但具体的分子机制尚不清楚。本研究的目的是探讨lncRNA TUG 1对肾I/R损伤中氧化应激和细胞凋亡的调节作用。本研究发现，在体外肾脏I/R损伤和缺氧/再灌注（H/R）损伤中，lncRNA TUG 1的表达水平上调，氧化应激水平和凋亡水平与lncRNA TUG 1的表达水平呈负相关。使用生物信息学数据库，如TargetScan和microRNA.org，预测microRNA-144 - 3 p（miR-144 - 3 p）参与lncRNA TUG 1和Nrf 2之间的关联。本研究证实，在体外肾I/R损伤和H/R损伤后，miR-144 - 3 p的水平显著降低，并且确定miR-144 - 3 p靶向Nrf 2并抑制其表达。此外，lncRNA TUG 1可以通过海绵状吸收miR-144 - 3 p来降低miR-144 - 3 p对Nrf 2的抑制作用。综上所述，我们的研究表明lncRNA TUG 1通过miR-144 - 3 p/Nrf 2轴调节肾I/R损伤过程中的氧化应激和细胞凋亡，这可能是肾I/R损伤的新治疗靶点。
Renal ischaemia/reperfusion (I/R) injury may induce kidney damage and dysfunction, in which oxidative stress and apoptosis play important roles. Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) are reported to be closely related to renal I/R, but the specific molecular mechanism is still unclear. The purpose of this research was to explore the regulatory effect of lncRNA TUG1 on oxidative stress and apoptosis in renal I/R injury. This research revealed that in renal I/R injury and hypoxia/reperfusion (H/R) injury in vitro, the expression level of lncRNA TUG1 was upregulated, and oxidative stress levels and apoptosis levels were negatively correlated with the expression level of lncRNA TUG1. Using bioinformatics databases such as TargetScan and microRNA.org, microRNA‐144‐3p (miR‐144‐3p) was predicted to be involved in the association between lncRNA TUG1 and Nrf2. This study confirmed that the level of miR‐144‐3p was significantly reduced following renal I/R injury and H/R injury in vitro, and miR‐144‐3p was determined to target Nrf2 and inhibit its expression. In addition, lncRNA TUG1 can reduce the inhibitory effect of miR‐144‐3p on Nrf2 by sponging miR‐144‐3p. In summary, our research shows that lncRNA TUG1 regulates oxidative stress and apoptosis during renal I/R injury through the miR‐144‐3p/Nrf2 axis, which may be a new treatment target for renal I/R injury.
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急性肾脏损伤的步枪标准与重症患者的医院死亡率有关：一项队列分析。
影响因子：
15.1
作者：
Hoste, Eric A. J.;Clermont, Gilles;Kersten, Alexander;Venkataraman, Ramesh;Angus, Derek C.;De Bacquer, Dirk;Kellum, John A.
通讯作者：
Kellum, John A.
DOI：
10.3390/molecules22030436
发表时间：
2017-03-09
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Fuse Y;Kobayashi M
通讯作者：
Kobayashi M
影响因子：
10.5
作者：
Itoh, K;Wakabayashi, N;Yamamoto, M
通讯作者：
Yamamoto, M
影响因子：
5.1
作者：
Anzell AR;Maizy R;Przyklenk K;Sanderson TH
通讯作者：
Sanderson TH
影响因子：
11.4
作者：
Joo Choi R;Cheng MS;Shik Kim Y
通讯作者：
Shik Kim Y