In search of cellular immunophenotypes in the blood of children with autism.
In search of cellular immunophenotypes in the blood of children with autism.
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DOI：
10.1371/journal.pone.0019299
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发表时间：
2011-05-04
期刊：
影响因子：
3.7
通讯作者：
Amaral DG
中科院分区：
文献类型：
--
作者：
Ashwood P;Corbett BA;Kantor A;Schulman H;Van de Water J;Amaral DG
自闭症是一种神经发育障碍，以社交行为障碍、沟通困难和重复或刻板行为的发生为特征。有大量证据表明自闭症患者的免疫系统调节失调。我们评估了自闭症幼儿(n = 70)与年龄匹配的对照组(n = 35)循环血细胞数量和表型的差异。确诊为自闭症的儿童(4-6岁)被进一步细分为低智商(IQ<；68，n = 35)组和高功能(IQ≥68，n = 35)组。年龄和性别匹配的典型发育中儿童为对照组。644个主要和次要变量，包括细胞计数和细胞表面抗原的丰度，用微体积激光扫描细胞术进行了评估。自闭症组和对照组之间的免疫细胞群有多种差异。孤独症患儿单位血B细胞绝对数高出20%以上，NK细胞绝对数高出40%左右。这两个变量在低功能自闭症组和高功能自闭症组之间都没有显著差异。虽然T细胞的绝对数量在不同组之间没有差异，但自闭症组的一些细胞激活标志，包括T细胞上的HLA-DR和CD26，以及B细胞上的CD38，明显高于对照组。这些结果支持了之前的发现，即一些自闭症儿童可能会发生免疫功能障碍。需要进一步评估功能障碍的性质，以及它如何在自闭症的病因学或自闭症的神经病理学和/或行为方面发挥作用。
Autism is a neurodevelopmental disorder characterized by impairments in social behavior, communication difficulties and the occurrence of repetitive or stereotyped behaviors. There has been substantial evidence for dysregulation of the immune system in autism. We evaluated differences in the number and phenotype of circulating blood cells in young children with autism (n = 70) compared with age-matched controls (n = 35). Children with a confirmed diagnosis of autism (4–6 years of age) were further subdivided into low (IQ<68, n = 35) or high functioning (IQ≥68, n = 35) groups. Age- and gender-matched typically developing children constituted the control group. Six hundred and forty four primary and secondary variables, including cell counts and the abundance of cell surface antigens, were assessed using microvolume laser scanning cytometry. There were multiple differences in immune cell populations between the autism and control groups. The absolute number of B cells per volume of blood was over 20% higher for children with autism and the absolute number of NK cells was about 40% higher. Neither of these variables showed significant difference between the low and high functioning autism groups. While the absolute number of T cells was not different across groups, a number of cellular activation markers, including HLA-DR and CD26 on T cells, and CD38 on B cells, were significantly higher in the autism group compared to controls. These results support previous findings that immune dysfunction may occur in some children with autism. Further evaluation of the nature of the dysfunction and how it may play a role in the etiology of autism or in facets of autism neuropathology and/or behavior are needed.
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影响因子：
9.1
作者：
Ashwood, P;Anthony, A;Wakefield, AJ
通讯作者：
Wakefield, AJ
影响因子：
3.3
作者：
Ashwood, P;Wakefield, AJ
通讯作者：
Wakefield, AJ
影响因子：
15.1
作者：
Enstrom, Amanda M.;Onore, Charity E.;de Water, Judy A. Van;Ashwood, Paul
通讯作者：
Ashwood, Paul
影响因子：
15.1
作者：
Enstrom, Amanda M.;Lit, Lisa;Onore, Charity E.;Gregg, Jeff P.;Hansen, Robin L.;Pessah, Isaac N.;Hertz-Picciotto, Irva;Van de Water, Judy A.;Sharp, Frank R.;Ashwood, Paul
通讯作者：
Ashwood, Paul
DOI：
10.1196/annals.1381.010
发表时间：
2007-01-01
期刊：
AUTOIMMUNITY, PT C
影响因子：
--
作者：
Cabanlit, Maricel;Wills, Sharifia;de Water, Judy Van
通讯作者：
de Water, Judy Van