Quantitative assessment of p16 expression in FNA specimens from head and neck squamous cell carcinoma and correlation with HPV status.
Quantitative assessment of p16 expression in FNA specimens from head and neck squamous cell carcinoma and correlation with HPV status.
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DOI：
10.1002/cncy.22399
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发表时间：
2021-05
影响因子：
3.4
通讯作者：
Adeniran AJ
中科院分区：
文献类型：
--
作者：
Abi-Raad R;Prasad ML;Gilani S;Garritano J;Barlow D;Cai G;Adeniran AJ
本研究应用免疫组化方法（IHC）检测头颈部鳞状细胞癌（HNSCC）细针穿刺标本中p16蛋白的表达，并应用聚合酶链反应（PCR）检测人乳头瘤病毒（HPV）的表达。采用受试者工作特征（ROC）曲线分析评估42例HNSCC患者手术标本中CB与p16 IHC的检测性能，以及细胞学标本中PCR与HPV的相关性。该研究通过PCR评估了FNA标本中的HPV，以替代手术标本中的p16 IHC。42例中，38例CB显示恶性细胞为具有或不具有单个肿瘤细胞的活细胞的粘附簇，而4例标本仅由单个肿瘤细胞和变性细胞组成。所有p16阴性的手术标本在相应的CB（n = 16）中均无p16染色。在p16阳性手术病例（n = 26）中，相应的肿瘤簇CB（n = 23）显示异质性p16表达，范围为40%至100%;然而，由于细胞降解，单细胞评分具有挑战性且不可靠。ROC曲线检验显示，最佳阈值为肿瘤簇中p16染色至少40%，敏感性和特异性均为100%。在CBs不足的病例中，通过PCR对针冲洗液进行HPV检测，对手术标本中p16表达的敏感性为88%，特异性为100%。肿瘤簇中至少40% p16表达的截止值可能适合于细胞学CB标本中的p16阳性。PCR检测针头冲洗液中HPV阳性可作为手术标本中p16表达的替代品。
This study investigated p16 by immunohistochemistry (IHC) on cellblocks (CBs) and human papillomavirus (HPV) by polymerase chain reaction (PCR) in fine-needle aspiration (FNA) of head and neck squamous cell carcinoma (HNSCC). Receiver operating characteristic (ROC) curve analysis was used to assess test performance in CBs compared with p16 IHC in 42 surgical specimens from patients with HNSCC and in correlation with HPV by PCR in cytology specimens. The study assessed HPV by PCR in FNA specimens as a substitute for p16 IHC in surgical specimens. Of 42 cases, 38 CBs showed malignant cells as cohesive clusters of viable cells with or without single tumor cells, whereas 4 specimens were composed exclusively of single tumor cells and degenerated cells. All p16-negative surgical specimens showed an absence of p16 staining in the corresponding CBs (n = 16). In the p16-positive surgical cases (n = 26), corresponding CBs with tumor clusters (n = 23) showed heterogeneous p16 expression ranging from 40% to 100%; however, scoring single cells was challenging and unreliable because of cellular degradation. ROC curve inspection showed the optimal threshold to be at least 40% p16 staining in tumor clusters with 100% sensitivity and specificity. In cases with inadequate CBs, HPV by PCR on needle rinse showed 88% sensitivity and 100% specificity for p16 expression in surgical specimens. A cutoff of at least 40% p16 expression in tumor clusters may be appropriate for p16 positivity in cytology CB specimens. A positive HPV finding by PCR on needle rinse can be used as a substitute for p16 expression in surgical specimens.
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影响因子：
2.6
作者：
Jakscha, Jens;Zlobec, Inti;Fischer, Claude A.
通讯作者：
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通讯作者：
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