Inner filter effect of gold nanoparticles on the fluorescence of rare-earth phosphate nanocrystals and its application for determination of biological aminothiols
Inner filter effect of gold nanoparticles on the fluorescence of rare-earth phosphate nanocrystals and its application for determination of biological aminothiols
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纳米金颗粒对稀土磷酸盐纳米晶荧光的内滤效应及其在生物氨基硫醇测定中的应用
DOI：
10.1016/j.jlumin.2013.03.021
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发表时间：
2013-09
影响因子：
3.6
通讯作者：
Wang, Lun
中科院分区：
文献类型：
--
作者：
Chen, Hong-Qi;Wu, Yong;Yuan, Fei;Xu, Juan;Zhang, Yi-Yan;Wang, Lun
基于内滤效应原理，以CePO 4：Tb 3+发光纳米粒子为供体，金纳米粒子（AuNPs）为能量受体，建立了一种简单、灵敏的生物氨基硫醇荧光检测方法。采用溶剂热法合成了稳定、水溶性好、分散性好的CePO 4：Tb 3+纳米粒子，并对其光漂白性能进行了研究。与此同时，具有高消光系数的金纳米颗粒预计能够作为强大的受体发挥作用。基于CePO 4：Tb 3+纳米粒子的发射光谱与Au纳米粒子的吸收光谱互补重叠，构建了内滤光器效应体系。在氨基硫醇（如半胱氨酸）的存在下，金纳米粒子与氨基硫醇相互作用，从而诱导金纳米粒子的聚集，这诱导了荧光恢复。本工作建立了一种测定生物氨基硫醇的开启荧光分析法。在最佳条件下，半胱氨酸、谷胱甘肽和同型半胱氨酸的线性范围分别为1.0×10−7-2.0×10− 6 M、5.0 ×10−8-5.0×10− 7 M和8.0×10−8-1.0×10− 6 M。该方法已成功地应用于合成样品中的生物氨基硫醇的定量。
A simple, sensitive fluorescent method for detecting biological aminothiols has been developed based on the inner filter effect principle that utilizes CePO4:Tb3+luminescent nanoparticles as the donor and gold nanoparticles (AuNPs) as the energy receptor. Stable, water-soluble and well-dispersible CePO4:Tb3+nanoparticles with low photobleaching features were synthesized conveniently by a facile solvothermal method. At the same time, AuNPs with a high extinction coefficient are expected to be capable of functioning as powerful receptor. Based on the complementary overlap between the emission spectrum of CePO4:Tb3+nanoparticles and the absorption spectrum of Au NPs, an inner filter effect system was constructed. In the presence of aminothiols (such as cysteine), AuNPs interacted with the aminothiols, thereby inducing the aggregation of AuNPs, which induced the fluorescence recovery. In the present work, we developed a turn-on fluorescent assay for the determination of biological aminothiols. Under the optimum conditions, the linear concentration ranges were 1.0×10−7–2.0×10−6M for cysteine, 5.0×10−8–5.0×10−7M for glutathione and 8.0×10−8–1.0×10−6M for homocysteine, respectively. The method is successfully applied to the quantification of biological aminothiols in synthetic samples.
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影响因子：
3.7
作者：
Guozhu Chen;Sixiu Sun;Wei Zhao;Shuling Xu;T. You
通讯作者：
Guozhu Chen;Sixiu Sun;Wei Zhao;Shuling Xu;T. You
影响因子：
4.2
作者：
Borisov, Sergey M.;Klimant, Ingo
通讯作者：
Klimant, Ingo
影响因子：
24.2
作者：
N. Tansil;Zhiqiang Gao
通讯作者：
N. Tansil;Zhiqiang Gao
影响因子：
7.4
作者：
GRABAR, KC;FREEMAN, RG;NATAN, MJ
通讯作者：
NATAN, MJ
影响因子：
4.2
作者：
Chen, Yi-Ming;Cheng, Tian-Lu;Tseng, Wei-Lung
通讯作者：
Tseng, Wei-Lung