Integrated Physiological, Transcriptomic, and Metabolomic Analyses Revealed Molecular Mechanism for Salt Resistance in Soybean Roots.
Integrated Physiological, Transcriptomic, and Metabolomic Analyses Revealed Molecular Mechanism for Salt Resistance in Soybean Roots.
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DOI：
10.3390/ijms222312848
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发表时间：
2021-11-27
影响因子：
5.6
通讯作者：
Bi Y
中科院分区：
文献类型：
--
作者：
Jin J;Wang J;Li K;Wang S;Qin J;Zhang G;Na X;Wang X;Bi Y
盐胁迫对包括大豆（Glycine max L.）在内的许多作物的产量构成威胁。本研究以3个抗盐性不同的大豆品种（JD 19、LH 3和LD 2）为材料，采用生理学、转录组学、代谢组学和生物信息学等方法对大豆耐盐机制进行了研究。生理研究表明，在100 mM NaCl胁迫下，耐盐品种JD 19和LH 3的根系生长抑制程度较低，抗氧化酶活性较高，活性氧积累量较低，Na+和Cl-含量较低。比较转录组分析表明，与LD 2相比，盐胁迫增加了JD 19和LH 3中抗氧化代谢、胁迫响应代谢、甘氨酸、丝氨酸和苏氨酸代谢、生长素响应蛋白、转录和抑制相关基因的表达。代谢产物谱的比较表明，氨基酸代谢和TCA循环是大豆响应盐胁迫的重要代谢途径。在对上述两条途径的进一步验证分析中发现，与LD 2相比，JD 19和LH 3在盐胁迫下具有更高的氮素吸收同化速率、更多的氨基酸积累和更快的TCA循环活性，有助于它们更好地适应盐胁迫。本研究为深入了解大豆耐盐性的分子机制提供了有价值的信息，也为培育耐盐大豆提供了新的思路和方法。
Salinity stress is a threat to yield in many crops, including soybean (Glycine max L.). In this study, three soybean cultivars (JD19, LH3, and LD2) with different salt resistance were used to analyze salt tolerance mechanisms using physiology, transcriptomic, metabolomic, and bioinformatic methods. Physiological studies showed that salt-tolerant cultivars JD19 and LH3 had less root growth inhibition, higher antioxidant enzyme activities, lower ROS accumulation, and lower Na+ and Cl- contents than salt-susceptible cultivar LD2 under 100 mM NaCl treatment. Comparative transcriptome analysis showed that compared with LD2, salt stress increased the expression of antioxidant metabolism, stress response metabolism, glycine, serine and threonine metabolism, auxin response protein, transcription, and translation-related genes in JD19 and LH3. The comparison of metabolite profiles indicated that amino acid metabolism and the TCA cycle were important metabolic pathways of soybean in response to salt stress. In the further validation analysis of the above two pathways, it was found that compared with LD2, JD19, and LH3 had higher nitrogen absorption and assimilation rate, more amino acid accumulation, and faster TCA cycle activity under salt stress, which helped them better adapt to salt stress. Taken together, this study provides valuable information for better understanding the molecular mechanism underlying salt tolerance of soybean and also proposes new ideas and methods for cultivating stress-tolerant soybean.
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DOI：
10.1186/s12284-017-0189-7
发表时间：
2018-01-25
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Casartelli A;Riewe D;Hubberten HM;Altmann T;Hoefgen R;Heuer S
通讯作者：
Heuer S
影响因子：
4.3
作者：
Guo, Yushuang;Huang, Changjun;Zhou, Xueping
通讯作者：
Zhou, Xueping
影响因子：
7.4
作者：
Jenner, HL;Winning, BM;Hill, SA
通讯作者：
Hill, SA
影响因子：
4.9
作者：
Chen, Taixiang;Johnson, Richard;Li, Chunjie
通讯作者：
Li, Chunjie
影响因子：
2.1
作者：
Botella, MA;Martinez, V;Cerda, A
通讯作者：
Cerda, A