A putative role for amino acid permeases in sink-source communication of barley tissues uncovered by RNA-seq.
A putative role for amino acid permeases in sink-source communication of barley tissues uncovered by RNA-seq.
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DOI：
10.1186/1471-2229-12-154
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发表时间：
2012-08-30
影响因子：
5.3
通讯作者：
Weschke W
中科院分区：
文献类型：
--
作者：
Kohl S;Hollmann J;Blattner FR;Radchuk V;Andersch F;Steuernagel B;Schmutzer T;Scholz U;Krupinska K;Weber H;Weschke W
谷物籽粒中积累的大部分氮来自营养器官中的蛋白质。然而，灌浆和再动员之间的相互作用知之甚少。我们使用转录组大规模焦磷酸测序的旗叶，颖壳和发展中的谷物，以确定半胱氨酸肽酶和N转运蛋白基因发挥作用的再动员和积累的氮在大麦。结合已知的和新获得的序列信息减少了冗余，增加了重叠群长度，并确定了半胱氨酸肽酶和N转运蛋白基因家族的新成员。利用Aramemnon数据库中的水稻和拟南芥的N转运蛋白氨基酸序列对N转运蛋白基因数据集进行比对。该方法共鉴定出57个AAT、45个NRT 1/PTR和22个OPT单基因，在系统发育树中各聚为一个亚群，其中25个AAT、8个NRT 1/PTR和5个OPT全长序列。此外，还鉴定了59个编码半胱氨酸肽酶的单基因，并将其细分为木瓜蛋白酶半胱氨酸肽酶进化枝的不同家族。全长AAT基因的表达谱突出显示氨基酸通透酶作为显示最高转录活性的组。HvAAP 2和HvAAP 6在营养器官中高度表达，而HvAAP 3是谷物特异性的。序列相似性聚类HvAAP 2和推定的转运蛋白HvAAP 6与拟南芥转运蛋白，这是参与氨基酸的长距离转移。HvAAP 3与AtAAP 1和AtAAP 8密切相关，它们在向发育中的种子提供N方面发挥作用。HvLHT 1和HvLHT 2在氨基酸重转运中起重要作用，它们分别在颖片和旗叶中特异性表达。主成分分析和K-均值聚类的AAT转录数据显示协调的发育阶段，剑叶，颖壳和籽粒。韧皮部特定的代谢化合物提出，可能信号高粮食需求的N到遥远的植物器官。该方法确定了半胱氨酸肽酶和特定的氮转运蛋白的AAT家族明显相关的籽粒灌浆，从而，在大麦的产量和品质。到目前为止，信息仅基于记录数据。为了使其与应用相关，必须更详细地分析已确定的候选人在汇-源通信中的作用。
The majority of nitrogen accumulating in cereal grains originates from proteins remobilised from vegetative organs. However, interactions between grain filling and remobilisation are poorly understood. We used transcriptome large-scale pyrosequencing of flag leaves, glumes and developing grains to identify cysteine peptidase and N transporter genes playing a role in remobilisation and accumulation of nitrogen in barley. Combination of already known and newly derived sequence information reduced redundancy, increased contig length and identified new members of cysteine peptidase and N transporter gene families. The dataset for N transporter genes was aligned with N transporter amino acid sequences of rice and Arabidopsis derived from Aramemnon database. 57 AAT, 45 NRT1/PTR and 22 OPT unigenes identified by this approach cluster to defined subgroups in the respective phylogenetic trees, among them 25 AAT, 8 NRT1/PTR and 5 OPT full-length sequences. Besides, 59 unigenes encoding cysteine peptidases were identified and subdivided into different families of the papain cysteine peptidase clade. Expression profiling of full-length AAT genes highlighted amino acid permeases as the group showing highest transcriptional activity. HvAAP2 and HvAAP6 are highly expressed in vegetative organs whereas HvAAP3 is grain-specific. Sequence similarities cluster HvAAP2 and the putative transporter HvAAP6 together with Arabidopsis transporters, which are involved in long-distance transfer of amino acids. HvAAP3 is closely related to AtAAP1 and AtAAP8 playing a role in supplying N to developing seeds. An important role in amino acid re-translocation can be considered for HvLHT1 and HvLHT2 which are specifically expressed in glumes and flag leaves, respectively. PCA and K-means clustering of AAT transcript data revealed coordinate developmental stages in flag leaves, glumes and grains. Phloem-specific metabolic compounds are proposed that might signal high grain demands for N to distantly located plant organs. The approach identified cysteine peptidases and specific N transporters of the AAT family as obviously relevant for grain filling and thus, grain yield and quality in barley. Up to now, information is based only on transcript data. To make it relevant for application, the role of identified candidates in sink-source communication has to be analysed in more detail.
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影响因子：
3.4
作者：
Martinez M;Diaz I
通讯作者：
Diaz I
影响因子：
4.3
作者：
Lopes, Marta S.;Cortadellas, Nuria;Araus, Jose L.
通讯作者：
Araus, Jose L.
影响因子：
9.4
作者：
Jukanti, Aravind K.;Heidlebaugh, Nancy M.;Fischer, Andreas M.
通讯作者：
Fischer, Andreas M.
影响因子：
11.4
作者：
Liu, D;Gong, W;Zhu, YX
通讯作者：
Zhu, YX
影响因子：
13.8
作者：
Gregersen, Per L.;Holm, Preben Bach
通讯作者：
Holm, Preben Bach