Expression of human leukocyte antigen G is associated with prognosis in nasopharyngeal carcinoma.
Expression of human leukocyte antigen G is associated with prognosis in nasopharyngeal carcinoma.
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DOI：
10.7150/ijbs.4383
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发表时间：
2012
影响因子：
9.2
通讯作者：
Zeng YX
中科院分区：
文献类型：
--
作者：
Cai MB;Han HQ;Bei JX;Liu CC;Lei JJ;Cui Q;Feng QS;Wang HY;Zhang JX;Liang Y;Chen LZ;Kang TB;Shao JY;Zeng YX
人类白细胞抗原 G (HLA-G) 具有多种免疫调节功能，包括诱导恶性肿瘤的免疫耐受。尚未研究 HLA-G 在鼻咽癌 (NPC) 中的作用。本研究旨在评价HLA-G作为鼻咽癌患者预后因素的作用及其在免疫调节中的作用。 Western检测显示鼻咽癌细胞系中HLA-G表达高，但永生化鼻咽上皮细胞系NP69中HLA-G表达低。免疫组织化学（IHC）进一步在552例鼻咽癌标本中检测到HLA-G蛋白，占79.2%，但在正常鼻咽上皮组织中未检测到HLA-G蛋白。此外，HLA-G的高表达预示着鼻咽癌患者的生存率较差，并且与肿瘤N分类和复发或转移呈正相关。多变量分析表明HLA-G是一个独立且不利的预后因素。此外，还检查了 CD68+巨噬细胞和 IL-10 的存在，它们是鼻咽癌的两个预后标志物，也是调节免疫监视的重要因素。在鼻咽癌组织中揭示了 HLA-G 与这两种免疫因子的相关性。综上所述，我们的结果表明 HLA-G 是鼻咽癌预后的独立生物标志物，HLA-G 可能有助于鼻咽癌的进展，可能与巨噬细胞和 IL-10 一起共同调节鼻咽癌的免疫监视。
Human leukocyte antigen G (HLA-G) has multiple immune regulatory functions including the induction of immune tolerance in malignancies. The roles of HLA-G have not been investigated in nasopharyngeal carcinoma (NPC). This study is aimed to evaluate the role of HLA-G as prognostic factor for NPC patients as well as its role in the immune regulation. Western assays showed high HLA-G expression in NPC cell lines, but low in the immortalized nasopharyngeal epithelial cell line NP69. HLA-G protein was further detected in 79.2% of 552 NPC specimens with immunohistochemistry (IHC), but not in normal nasopharyngeal epithelium tissue. Moreover, high expression of HLA-G predicted poor survival of NPC patients and positively correlated with tumor N classification and recurrence or metastasis. Multivariate analysis indicated that HLA-G was an independent and unfavorable prognostic factor. Furthermore, the presence of CD68+macrophages and IL-10 were also examined, which are two prognostic markers of NPC and important factors for regulating immune surveillance. The correlations of HLA-G with these two immune factors were revealed in NPC tissues. Taken together, our results suggest that HLA-G is an independent biomarker for NPC prognosis, and HLA-G might contribute to NPC progression, which might jointly regulate immune surveillance in NPC together with macrophages and IL-10.
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DOI：
10.1084/jem.184.2.539
发表时间：
1996-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Roth I;Corry DB;Locksley RM;Abrams JS;Litton MJ;Fisher SJ
通讯作者：
Fisher SJ
影响因子：
7.7
作者：
Cirulli, V;Zalatan, J;Crisa, L
通讯作者：
Crisa, L
影响因子：
5
作者：
Antonia Rodriguez, Josefa;Galeano, Liliana;Lucia Combita, Alba
通讯作者：
Lucia Combita, Alba
影响因子：
2.4
作者：
Özyar, E;Ayhan, A;Atahan, IL
通讯作者：
Atahan, IL
影响因子：
4.7
作者：
Barrier, Breton F.;Kendall, Brian S.;Kost, Edward R.
通讯作者：
Kost, Edward R.