Temephos resistance in Aedes aegypti in Colombia compromises dengue vector control.
Temephos resistance in Aedes aegypti in Colombia compromises dengue vector control.
复制标题
DOI：
10.1371/journal.pntd.0002438
复制
发表时间：
2013
影响因子：
3.8
通讯作者：
Lenhart A
中科院分区：
文献类型：
--
作者：
Grisales N;Poupardin R;Gomez S;Fonseca-Gonzalez I;Ranson H;Lenhart A
登革热的控制和预防在很大程度上依赖于杀虫剂的应用来控制登革热媒介蚊子。在哥伦比亚，在埃及伊蚊的水生繁殖地使用杀幼虫剂双硫磷是登革热控制策略的关键部分。最近在登革热流行城市库库塔发现了对替美福的耐药性，引发了人们对其作为控制工具的功效的质疑。在这里，我们描述了潜在机制并估计了这种阻力的操作影响。 Ae 幼虫生物测定。来自 Cucuta 的埃及伊蚊幼虫测得的替美磷 LC50 为 0.066 ppm (95% CI 0.06–0.074)，比从易感实验室菌落获得的值高出约 15 倍。田间剂量的替美磷杀死这种抗性库库塔种群的功效大大降低，施用两周后死亡率<80%，4周后死亡率<50%。 ace-1 基因的生化测定和部分测序均未表明靶位点抗性是主要抗性机制。协同作用测定和微阵列分析表明代谢机制很可能是导致替美磷耐药的原因。有趣的是，虽然用羧酸酯酶抑制剂 DEF 观察到最大的协同作用，但微阵列分析中并通过定量 PCR 证实的主要候选基因是细胞色素 P450 氧化酶，特别是 CYP6N12、CYP6F3 和 CYP6M11。在哥伦比亚，埃及伊蚊对替美磷产生了抗药性。埃及伊蚊会影响其作为病媒控制工具的效果持续时间。确定了几个可能与替美磷代谢抗性有关的候选基因。鉴于批准用于病媒控制的杀虫剂数量有限，未来基于化学的控制策略应考虑抗性背后的机制，以确定哪些杀虫剂可能会带来最大的控制效果，同时最大限度地减少抗性表型的进一步选择。登革热由埃及伊蚊传播的病毒引起，对许多热带和亚热带国家的公共卫生构成重要威胁。在缺乏疫苗或特定药物治疗的情况下，登革热传播的预防和控制依赖于针对病媒蚊群的干预措施。在哥伦比亚库库塔市，数十年来一直使用杀虫剂替美磷来控制伊蚊。埃及幼虫，直到最近报道了耐药性。在这项研究中，量化了该人群对替美磷的抗性，并通过模拟田间试验估计了其对防治活动的影响。耐药性的机制被确定为代谢性的，几种关键的解毒酶被确定为潜在的候选酶。在哥伦比亚该地区制定未来病媒控制和杀虫剂抗性管理战略时应考虑到这一点。
Control and prevention of dengue relies heavily on the application of insecticides to control dengue vector mosquitoes. In Colombia, application of the larvicide temephos to the aquatic breeding sites of Aedes aegypti is a key part of the dengue control strategy. Resistance to temephos was recently detected in the dengue-endemic city of Cucuta, leading to questions about its efficacy as a control tool. Here, we characterize the underlying mechanisms and estimate the operational impact of this resistance. Larval bioassays of Ae. aegypti larvae from Cucuta determined the temephos LC50 to be 0.066 ppm (95% CI 0.06–0.074), approximately 15× higher than the value obtained from a susceptible laboratory colony. The efficacy of the field dose of temephos at killing this resistant Cucuta population was greatly reduced, with mortality rates <80% two weeks after application and <50% after 4 weeks. Neither biochemical assays nor partial sequencing of the ace-1 gene implicated target site resistance as the primary resistance mechanism. Synergism assays and microarray analysis suggested that metabolic mechanisms were most likely responsible for the temephos resistance. Interestingly, although the greatest synergism was observed with the carboxylesterase inhibitor, DEF, the primary candidate genes from the microarray analysis, and confirmed by quantitative PCR, were cytochrome P450 oxidases, notably CYP6N12, CYP6F3 and CYP6M11. In Colombia, resistance to temephos in Ae. aegypti compromises the duration of its effect as a vector control tool. Several candidate genes potentially responsible for metabolic resistance to temephos were identified. Given the limited number of insecticides that are approved for vector control, future chemical-based control strategies should take into account the mechanisms underlying the resistance to discern which insecticides would likely lead to the greatest control efficacy while minimizing further selection of resistant phenotypes. Dengue fever, caused by viruses transmitted by the Aedes aegypti mosquito, is an important threat to public health in many tropical and subtropical countries. In the absence of a vaccine or specific drug treatment, prevention and control of dengue transmission relies on interventions targeting vector mosquito populations. In the city of Cucuta, Colombia, the insecticide temephos was used for several decades to control Ae. aegypti larvae, until resistance was recently reported. In this study, the resistance to temephos in this population was quantified, and its impact on control activities estimated using simulated field trials. The mechanisms underlying the resistance were determined to be metabolic, with several key detoxification enzymes identified as potential candidates. This should be taken into account when devising future vector control and insecticide resistance management strategies in this region of Colombia.
登录
查看更多内容
DOI：
10.1590/s0074-02762010000100019
发表时间：
2010-02-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Llinás, G Albrieu;Seccacini, E;Licastro, S
通讯作者：
Licastro, S
影响因子：
4.4
作者：
David, Jean-Philippe;Coissac, Eric;Reynaud, Stephane
通讯作者：
Reynaud, Stephane
影响因子：
2.6
作者：
Assis de Carvalho, Renato;Torres, Tatiana Teixeira;Lima de Azeredo-Espin, Ana Maria
通讯作者：
Lima de Azeredo-Espin, Ana Maria
影响因子：
4.8
作者：
Cui, Feng;Qu, Hong;Qiao, Chuan-Ling
通讯作者：
Qiao, Chuan-Ling
影响因子：
2.7
作者：
Lenhart, AE;Walle, M;Kroeger, A
通讯作者：
Kroeger, A