Immunocytochemical evidence of the localization of the Crumbs homologue 3 protein (CRB3) in the developing and mature mouse retina.
Immunocytochemical evidence of the localization of the Crumbs homologue 3 protein (CRB3) in the developing and mature mouse retina.
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DOI：
10.1371/journal.pone.0050511
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lillo C
中科院分区：
文献类型：
--
作者：
Herranz-Martín S;Jimeno D;Paniagua AE;Velasco A;Lara JM;Aijón J;Lillo C
CRB3 （crumbhomologue 3）是CRB蛋白家族的一员（与果蝇的crumbhomologue 3同源），在哺乳动物的不同上皮源性细胞类型中表达，似乎参与调节紧密连接的建立和稳定性以及纤毛发生。这种蛋白在视网膜中也被检测到，但对其在视网膜组织中的定位和功能知之甚少。我们的目的是深入研究CRB3蛋白在小鼠视网膜中的存在，并分析其在光感受器纤毛形成和丛状视网膜层建立过程中的表达。采用双免疫荧光实验对CRB3和已知的不同视网膜细胞类型标记物进行定位研究。根据我们的研究结果，CRB3从出生后0天（P0）一直存在于小鼠视网膜中直到成年。它定位于光感受器细胞的内节（is），尤其集中在连接纤毛所在的区域，光感受器细胞外丛状层（OPL）的突触终末，以及内丛状层（IPL）的无突细胞和双极细胞亚群中。
CRB3 (Crumbs homologue 3), a member of the CRB protein family (homologous to the Drosophila Crumbs), is expressed in different epithelium-derived cell types in mammals, where it seems to be involved in regulating the establishment and stability of tight junctions and in ciliogenesis. This protein has been also detected in the retina, but little is known about its localization and function in this tissue. Our goal here was to perform an in-depth study of the presence of CRB3 protein in the mouse retina and to analyze its expression during photoreceptor ciliogenesis and the establishment of the plexiform retinal layers. Double immunofluorescence experiments for CRB3 and well-known markers for the different retinal cell types were performed to study the localization of the CRB3 protein. According to our results, CRB3 is present from postnatal day 0 (P0) until adulthood in the mouse retina. It is localized in the inner segments (IS) of photoreceptor cells, especially concentrated in the area where the connecting cilium is located, in their synaptic terminals in the outer plexiform layer (OPL), and in sub-populations of amacrine and bipolar cells in the inner plexiform layer (IPL).
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影响因子：
3.3
作者：
Lemmers, C;Michel, D;Le Bivic, A
通讯作者：
Le Bivic, A
影响因子：
2.6
作者：
den Hollander, AI;Ghiani, M;Broccoli, V
通讯作者：
Broccoli, V
DOI：
10.1083/jcb.200609096
发表时间：
2007-07-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fan S;Fogg V;Wang Q;Chen XW;Liu CJ;Margolis B
通讯作者：
Margolis B
影响因子：
3.5
作者：
Evans, R. Jane;Schwarz, Nele;Cheetham, Michael E.
通讯作者：
Cheetham, Michael E.
影响因子：
3.3
作者：
Haverkamp, S.;Inta, D.;Waessle, H.
通讯作者：
Waessle, H.