microRNA Expression Profile of Purified Alveolar Epithelial Type II Cells.
microRNA Expression Profile of Purified Alveolar Epithelial Type II Cells.
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DOI：
10.3390/genes13081420
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发表时间：
2022-08-10
期刊：
影响因子：
3.5
通讯作者：
Krauss-Etschmann, Susanne
中科院分区：
文献类型：
--
作者：
Dehmel, Stefan;Weiss, Katharina J.;El-Merhie, Natalia;Callegari, Jens;Konrad, Birte;Mutze, Kathrin;Eickelberg, Oliver;Konigshoff, Melanie;Krauss-Etschmann, Susanne
关键词：
肺泡II型（ATII）细胞对于维持肺泡内环境稳定是必不可少的。然而，关于控制稳态和协调鼠ATII细胞的多种功能的miRNA和miRNA调节的网络的表达的知识是有限的。因此，我们想知道ATII细胞中表达的miRNAs如何有助于调节信号通路。我们使用具有高度自发荧光的肺细胞群的流式细胞术分选方法纯化"未被抗体接触"的ATII细胞。在分选的细胞上进行TaqMan® miRNA低密度阵列，并用根据先前公开的方案分离的ATII细胞的miRNA谱进行测序。在两种ATII制剂中表达的293种miRNA中，111种显示出相等的丰度。将真正的ATII miRNA的靶mRNA用于途径富集分析。该分析鉴定了在纤维化和/或上皮-间充质转化（EMT）中具有已知功能的9种信号传导途径。特别是，发现19种miRNA的子集靶向TGF-β信号通路的21种组分。在人A549细胞中，TGF-β 1刺激下调了其中三种miRNAs（miR-16 - 5p、-17-5p和-30 c-5p），并观察到相关mRNA靶点（BMPR 2、JUN、RUNX2）的伴随上调。这些结果表明，在生理条件下，miRNAs在维持ATII细胞中TGF-β信号通路的稳态中具有重要作用。
Alveolar type II (ATII) cells are essential for the maintenance of the alveolar homeostasis. However, knowledge of the expression of the miRNAs and miRNA-regulated networks which control homeostasis and coordinate diverse functions of murine ATII cells is limited. Therefore, we asked how miRNAs expressed in ATII cells might contribute to the regulation of signaling pathways. We purified “untouched by antibodies” ATII cells using a flow cytometric sorting method with a highly autofluorescent population of lung cells. TaqMan® miRNA low-density arrays were performed on sorted cells and intersected with miRNA profiles of ATII cells isolated according to a previously published protocol. Of 293 miRNAs expressed in both ATII preparations, 111 showed equal abundances. The target mRNAs of bona fide ATII miRNAs were used for pathway enrichment analysis. This analysis identified nine signaling pathways with known functions in fibrosis and/or epithelial-to-mesenchymal transition (EMT). In particular, a subset of 19 miRNAs was found to target 21 components of the TGF-β signaling pathway. Three of these miRNAs (miR-16-5p, -17-5p and -30c-5p) were down-modulated by TGF-β1 stimulation in human A549 cells, and concomitant up-regulation of associated mRNA targets (BMPR2, JUN, RUNX2) was observed. These results suggest an important role for miRNAs in maintaining the homeostasis of the TGF-β signaling pathway in ATII cells under physiological conditions.
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3.5
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发表时间：
2009-03
期刊：
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影响因子：
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作者：
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影响因子：
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