Mechanical Response of Neural Cells to Physiologically Relevant Stiffness Gradients.
Mechanical Response of Neural Cells to Physiologically Relevant Stiffness Gradients.
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DOI：
10.1002/adhm.201901036
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发表时间：
2020-04
影响因子：
10
通讯作者：
Phillips JB
中科院分区：
文献类型：
--
作者：
Kayal C;Moeendarbary E;Shipley RJ;Phillips JB
了解机械环境对神经突行为的影响对于周围神经修复解决方案的开发至关重要，并且可以帮助组织工程师指导和引导再生。在这项研究中，提出了一种新的协议，以制造生理相关的水凝胶基板与控制机械线索。这些水凝胶允许分析绝对刚度值和局部刚度梯度对神经细胞行为的相对影响，特别是对于低刚度值（1- 2kPa）。NG 108 - 15神经细胞行为研究使用了良好表征的胶原梯度基质，刚度值范围为1至10 kPa，梯度斜率为0.84或7.9 kPa mm−1。研究发现，单元取向受刚度值和刚度梯度的特定组合的影响。结果突出了考虑水凝胶的类型以及刚度的绝对值和其梯度的陡度的重要性，从而为组织工程支架的开发和基底刚度的研究引入了新的框架。一个新的协议，以制造一个可重复的和机械特性的胶原蛋白为基础的模型的刚度梯度，有价值的硬旋转的研究，证明。定义的刚度梯度配置文件下的神经细胞的形态学响应进行了研究，并首次强调了局部刚度大小和梯度陡度的综合影响。这对理解神经细胞行为有重大贡献，并将为未来神经修复策略的改进奠定基础。
Understanding the influence of the mechanical environment on neurite behavior is crucial in the development of peripheral nerve repair solutions, and could help tissue engineers to direct and guide regeneration. In this study, a new protocol to fabricate physiologically relevant hydrogel substrates with controlled mechanical cues is proposed. These hydrogels allow the analysis of the relative effects of both the absolute stiffness value and the local stiffness gradient on neural cell behavior, particularly for low stiffness values (1–2 kPa). NG108‐15 neural cell behavior is studied using well‐characterized collagen gradient substrates with stiffness values ranging from 1 to 10 kPa and gradient slopes of either 0.84 or 7.9 kPa mm−1. It is found that cell orientation is influenced by specific combinations of stiffness value and stiffness gradient. The results highlight the importance of considering the type of hydrogel as well as both the absolute value of the stiffness and the steepness of its gradient, thus introducing a new framework for the development of tissue engineered scaffolds and the study of substrate stiffness. A new protocol to fabricate a reproducible and mechanically characterized collagen based model of stiffness gradients, valuable for the study of durotaxis, is demonstrated. Morphological responses of neural cells under defined stiffness gradient profiles are investigated and for the first time the combined influence of both local stiffness magnitude and the gradient steepness is highlighted. This contributes significantly to the understanding of neural cell behavior and will underpin future improvements to nerve repair strategies.
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影响因子：
25
作者：
Koser DE;Thompson AJ;Foster SK;Dwivedy A;Pillai EK;Sheridan GK;Svoboda H;Viana M;Costa LD;Guck J;Holt CE;Franze K
通讯作者：
Franze K
DOI：
10.1073/pnas.1611324113
发表时间：
2016-10-04
影响因子：
11.1
作者：
Hartman, Christopher D.;Isenberg, Brett C.;Wong, Joyce Y.
通讯作者：
Wong, Joyce Y.
影响因子：
14
作者：
Kloxin, April M.;Benton, Julie A.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
影响因子：
3.4
作者：
Isenberg, Brett C.;DiMilla, Paul A.;Wong, Joyce Y.
通讯作者：
Wong, Joyce Y.
在锯状的微弹性水凝胶上，带有不对称梯度棘轮齿的锯状细胞迁移。
影响因子：
3.7
作者：
Kidoaki S;Sakashita H
通讯作者：
Sakashita H