Rectified cell migration on saw-like micro-elastically patterned hydrogels with asymmetric gradient ratchet teeth.
Rectified cell migration on saw-like micro-elastically patterned hydrogels with asymmetric gradient ratchet teeth.
复制标题
在锯状的微弹性水凝胶上，带有不对称梯度棘轮齿的锯状细胞迁移。
DOI：
10.1371/journal.pone.0078067
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sakashita H
中科院分区：
文献类型：
--
作者：
Kidoaki S;Sakashita H
控制细胞运动是生物医学工程的关键技术之一。为了建立一种弹性基底表面设计的方法来控制远程细胞运动，在这里，我们报告了一个复杂的细胞培养水凝胶与微弹性图案化的表面，允许远程硬旋转。这种水凝胶具有带有不对称梯度棘齿的锯齿状图案，并纠正随机细胞运动。趋硬性仅发生在弹性梯度强度高于阈值水平的边界处。因此，在具有单位齿图案的凝胶中，硬旋转应仅发生在弹性梯度强度高于该阈值水平的牙齿侧。因此，预期这种凝胶通过类似于Feynman-Smoluchowski棘轮的机制支持细胞的长距离偏向运动，即，纠正细胞迁移。本研究验证了这一工作假设，通过使用微弹性图案的光固化明胶凝胶。凝胶，其中每个牙齿单元为100-120 µm宽，上升：下降弹性梯度的比例为1：2，峰值弹性约为100 - 120 µ m。100 kPa支持3 T3成纤维细胞的有效整流迁移。此外，当软泳道垂直于锯齿状图案引入时，长距离细胞迁移是最有效的。这项研究表明，细胞培养凝胶的不对称弹性梯度图案化是一种操纵细胞运动的通用手段。
To control cell motility is one of the essential technologies for biomedical engineering. To establish a methodology of the surface design of elastic substrate to control the long-range cell movements, here we report a sophisticated cell culture hydrogel with a micro-elastically patterned surface that allows long-range durotaxis. This hydrogel has a saw-like pattern with asymmetric gradient ratchet teeth, and rectifies random cell movements. Durotaxis only occurs at boundaries in which the gradient strength of elasticity is above a threshold level. Consequently, in gels with unit teeth patterns, durotaxis should only occur at the sides of the teeth in which the gradient strength of elasticity is above this threshold level. Therefore, such gels are expected to support the long-range biased movement of cells via a mechanism similar to the Feynman-Smoluchowski ratchet, i.e., rectified cell migration. The present study verifies this working hypothesis by using photolithographic microelasticity patterning of photocurable gelatin gels. Gels in which each teeth unit was 100–120 µm wide with a ratio of ascending:descending elasticity gradient of 1:2 and a peak elasticity of ca. 100 kPa supported the efficient rectified migration of 3T3 fibroblast cells. In addition, long-range cell migration was most efficient when soft lanes were introduced perpendicular to the saw-like patterns. This study demonstrates that asymmetric elasticity gradient patterning of cell culture gels is a versatile means of manipulating cell motility.
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