Feminization of the hepatic microsomal cytochrome P-450 system in brook trout by estradiol, testosterone, and pituitary factors.
Feminization of the hepatic microsomal cytochrome P-450 system in brook trout by estradiol, testosterone, and pituitary factors.
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雌二醇、睾酮和垂体因子导致鳟鱼肝微粒体细胞色素 P-450 系统女性化。
DOI：
10.1002/jez.1402530108
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Woodin,BR
中科院分区：
文献类型：
--
作者：
Pajor,AM;Stegeman,JJ;Thomas,P;Woodin,BR
在假手术和性腺切除的未成熟溪鳟中研究了雌二醇、睾酮和垂体提取物对肝微粒体酶的影响。在两组中，雌二醇使细胞色素P-450和b5的比含量降低了70%或更多。睾酮和垂体提取物也降低了总P-450和b5的水平，但程度较小。当P-450和b5含量标准化/g肝脏时，后一种效应不明显。用来自硬骨鱼（scup）的P-450形式的抗体进行的免疫印迹分析显示，对照鱼中存在交叉反应蛋白，推测与scup形式对应。雌激素处理的鱼中，鳟鱼对应于P-450 A（一种假定的睾酮6β-羟化酶）的水平受到强烈抑制。鳟鱼P-450 B对应物在雌二醇处理的鱼中受到抑制，但不如P-450 A对应物那么强烈。烃诱导型P-450 E（P-450 IA 1）的鳟鱼直系同源物，芳烃羟化酶（AHH）催化剂，在任何组中都检测不到，这与这些鱼中非常低的AHH活性水平一致。雌二醇或垂体提取物也降低了假手术鱼的NADH-细胞色素b5和NADPH-细胞色素P-450还原酶活性水平，但在性腺切除的鱼中没有。单独的性腺切除术也降低了还原酶活性的水平，但没有降低总P-450或b5的水平，这表明性腺在维持还原酶的正常水平方面有影响。结果支持雌激素在产生鱼肝微粒体酶的一般性别差异方面具有显著的抑制作用，并表明可能对几种P-450形式产生影响。然而，激素在这种调节中的作用机制尚不确定。结果还表明，存在身份不明，可能是性腺，因素，这有助于调节还原酶在硬骨鱼肝脏。
The effects of estradiol, testosterone, and pituitary extract on hepatic microsomal enzymes were studied in sham‐operated and gonadectomized immature brook trout. Estradiol reduced the specific content of cytochromes P‐450 and b5by 70% or more in both groups. Testosterone and pituitary extract also decreased the levels of total P‐450 and b5, but to a lesser extent. These latter effects were not evident when the contents of P‐450 and b5were normalized per g liver. Immunoblot analysis with antibodies to P‐450 forms from a teleost (scup) showed the presence of cross‐reacting proteins in control fish, presumed counterparts to the scup forms. Levels of a trout counterpart to P‐450A (a putative testosterone 6β‐hydroxylase) were strongly suppressed in estradiol‐treated fish. A trout P‐450B counterpart was suppressed in estradiol‐treated fish, but less strongly than was the P‐450A counterpart. The trout orthologue of hydrocarbon‐inducible P‐450E (P‐450IA1), the aryl hydrocarbon hydroxylase (AHH) catalyst, was undetectable in any group, consistent with very low levels of AHH activity in these fish. Estradiol or pituitary extract also decreased the levels of NADH‐cytochrome b5and NADPH‐cytochrome P‐450 reductase activities in sham‐operated but not in gonadectomized fish. Gonadectomy alone also lowered the levels of reductase activities but not of total P‐450 or b5, suggesting an influence of gonads in maintaining normal levels of the reductases. The results support a prominent suppressive role for estrogens in producing the general sex differences in microsomal enzymes in fish liver and indicate that there could be affects on several P‐450 forms. However, the mechanism of hormone action in this regulation is uncertain. The results also suggest the existence of unidentified, possibly gonadal, factors which contribute to the regulation of reductases in teleost liver.
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虹鳟鱼 4-雄烯-3,17-二酮肝脏体外代谢的性别差异。
影响因子：
2.7
作者：
T. Hansson;J. Gustafsson
通讯作者：
J. Gustafsson
异斜眼底诱导细胞色素 P-450 与多氯联苯和多核芳烃环境污染相关
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
A. Elskus;J. Stegeman
通讯作者：
J. Stegeman
雌激素刺激后鸡肝微粒体的化学和酶促变化。
DOI：
10.1111/j.1432-0436.1974.tb00351.x
发表时间：
1974
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
O. Schjeide;R. Prince;T. Nicholls;B. Wanamacher
通讯作者：
B. Wanamacher
生长激素在翻译前水平调节大鼠肝细胞色素 P-450 15 beta 的表达。
DOI：
10.1016/0006-291x(87)91422-7
发表时间：
1987
影响因子：
3.1
作者：
C. Macgeoch;E. Morgan;B. Cordell;J. Gustafsson
通讯作者：
J. Gustafsson
影响因子：
3.9
作者：
KLOEPPERSAMS, PJ;PARK, SS;STEGEMAN, JJ
通讯作者：
STEGEMAN, JJ