A multimode optical imaging system for preclinical applications in vivo: technology development, multiscale imaging, and chemotherapy assessment.
A multimode optical imaging system for preclinical applications in vivo: technology development, multiscale imaging, and chemotherapy assessment.
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DOI：
10.1007/s11307-011-0517-z
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发表时间：
2012-08
影响因子：
3.1
通讯作者：
Farkas, Daniel L.
中科院分区：
文献类型：
--
作者：
Hwang, Jae Youn;Wachsmann-Hogiu, Sebastian;Ramanujan, V. Krishnan;Ljubimova, Julia;Gross, Zeev;Gray, Harry B.;Medina-Kauwe, Lali K.;Farkas, Daniel L.
关键词：
几种已建立的光学成像方法通常单独应用于临床前研究；然而，真正有用的体内成像可能需要同时组合成像方式来检查细胞和组织的动态特征。我们开发了一种新型多模式光学成像系统，旨在实现多种应用，产生高灵敏度和特异性的分子成像。我们将多种光学成像技术（包括荧光强度、光谱、寿命、活体共焦、双光子激发和生物发光）集成到一个系统中，从而在动物模型中实现功能性多尺度成像。该方法提供了一个全面的成像平台，用于对高度相关的信息进行动力学、定量和环境分析，具有微观到宏观的分辨率。应用于小动物体内，这提供了对感兴趣过程的卓越监测，此处以化学/纳米结构治疗评估为代表。这个新系统用途广泛，可以针对各种应用进行优化，其中重点是癌症检测和靶向治疗。
Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.
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DOI：
10.1155/dte.5.85
发表时间：
1999-01-01
期刊：
Diagnostic and therapeutic endoscopy
影响因子：
--
作者：
Ikeda, N;Honda, H;Ebihara, Y
通讯作者：
Ebihara, Y
影响因子：
2.5
作者：
Levenson, Richard M.;Lynch, David T.;Backer, Marina V.
通讯作者：
Backer, Marina V.
影响因子：
82.9
作者：
Falati, S;Gross, P;Furie, B
通讯作者：
Furie, B
影响因子：
2.9
作者：
Kocisov치, E;Praus, P;Turpin, PY
通讯作者：
Turpin, PY
影响因子：
2.5
作者：
Evans, JA;Nishioka, NS
通讯作者：
Nishioka, NS