Investigation of ANGPTL3 expression, exon sequence and promotor methylation between Ningxiang and Changbai pigs
Investigation of ANGPTL3 expression, exon sequence and promotor methylation between Ningxiang and Changbai pigs
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宁乡猪和长白猪ANGPTL3表达、外显子序列及启动子甲基化的研究
DOI：
10.4314/ahs.v12i4.20
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发表时间：
2013-02
影响因子：
1
通讯作者：
孙志良
中科院分区：
文献类型：
--
作者：
张大生;刘兆颖;陈小军;匡光伟;潘任桃;孙志良
背景：血管生成素样蛋白3(Angptl3)是血管生成素样蛋白中的一员，已被证明通过抑制脂蛋白脂酶(LPL)的活性来影响脂质代谢。目的：比较宁乡猪和长白猪血管紧张素转换酶3基因的表达、外显子突变和启动子区CpG岛甲基化状态。方法：屠宰猪，采集约100 mg组织样品和皮下脂肪组织进行分析。进行实时定量聚合酶链式反应、Western blotting、外显子测序和HRM分析。结果：Angptl3在肝脏有表达，在脂肪和瘦肉中无表达。与长白猪相比，宁乡猪的Angptl3mRNA水平较低。然而，这两组猪之间的蛋白表达没有差异。序列分析表明，长白猪与宁乡猪之间存在4个氨基酸变异，其中3个变异不引起氨基酸变化，1个变异引起Val(长白)到Met(宁乡)氨基酸突变。测序结果表明，长白猪和宁乡猪的平均甲基化率在70.952%~95.238%之间。结论：这些结果支持长白猪和宁乡猪在高分辨熔融(HRM)分析中的显著差异(p<；0.05)。这些结果可能有助于更好地理解Angptl3在脂代谢中的作用。关键词：血管生成素样蛋白3，DNA甲基化，猪
Background: Angiopoietin-like protein 3 (ANGPTL3), a member of angiopoietin-like proteins, has been demonstrated to affect lipid metabolism by inhibiting the activity of lipoprotein lipase (LPL). Objective: To compare the ANGPTL3 mRNA and protein expression, exon mutation and promoter district CpG island methylation state between Ningxiang Pigs and Changbai Pigs. Methods: Pigs were slaughtered and about 100 mg of tissue samples and subcutaneous adipose tissue were collected and stored for analysis. Quantitative Real-Time PCR, Western blotting, exons sequencing, and HRM analysis were carried out. Results: ANGPTL3 was expressed in liver but not in fat and lean meat. Compared with Changbai pigs, ANGPTL3 mRNA level in Ningxiang pigs was lower. However, the protein expression showed no difference between these two groups of pigs. Sequences analysis showed that four variations existed between Changbai Pigs and Ningxiang Pigs, among which three variations caused no change of amino acids, and the other one caused amino acid mutation from Val (Changbai) to Met (Ningxiang). The ANGPTL3 promoter district CpG island bisulfite-PCR and sequencing results showed that the mean methylation rate ranged from 70.952% to 95.238% between Changbai pig and Ningxiang pig (p<0.05). Conclusion: These results support the significant difference (p<0.05) between Changbai pig and Ningxiang pig in highresolution melting (HRM) analysis. All these results may be helpful for a better understanding of the role of ANGPTL3 in lipid metabolism. Key words: Angiopoietin-like protein 3 (ANGPTL3), DNA methylation, pigs
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影响因子：
5.8
作者：
Legry, Vanessa;Bokor, Szilvia;Meirhaeghe, Aline
通讯作者：
Meirhaeghe, Aline
影响因子：
30.8
作者：
Koishi, R;Ando, Y;Furukawa, H
通讯作者：
Furukawa, H
DOI：
10.1016/j.ejps.2012.02.001
发表时间：
2012-05
期刊：
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
影响因子：
--
作者：
Hong-Bo Xiao;Zhi-Liang Sun;N. Zhou
通讯作者：
Hong-Bo Xiao;Zhi-Liang Sun;N. Zhou
影响因子：
5
作者：
Martin-Campos, Jesus M.;Roig, Rosa;Blanco-Vaca, Francisco
通讯作者：
Blanco-Vaca, Francisco
影响因子：
1.7
作者：
Feng, S. Q.;Chen, X. D.;Yang, Z. Q.
通讯作者：
Yang, Z. Q.