Real-Time Monitoring and Analysis of Zebrafish Electrocardiogram with Anomaly Detection.
Real-Time Monitoring and Analysis of Zebrafish Electrocardiogram with Anomaly Detection.
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DOI：
10.3390/s18010061
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发表时间：
2017-12-28
期刊：
影响因子：
--
通讯作者：
Cao H
中科院分区：
文献类型：
--
作者：
Lenning M;Fortunato J;Le T;Clark I;Sherpa A;Yi S;Hofsteen P;Thamilarasu G;Yang J;Xu X;Han HD;Hsiai TK;Cao H
心脏病是美国死亡的主要原因，每年约有61万人死亡。许多心脏疾病缺乏有效的治疗方法，主要是由于对其遗传基础和潜在分子机制的不完全理解。斑马鱼（Danio rerio）是研究心脏病的一个很好的模型系统，因为它们可以通过正向遗传方法来解决这种未满足的医疗需求。近年来，我们的团队一直采用心电图（ECG）作为一种有效的工具来研究斑马鱼心脏沿着与传统的方法，如免疫组织化学，DNA和蛋白质分析。为了获得具有良好信噪比（SNR）、高空间和时间分辨率的心电信号，我们克服了斑马鱼体型小和水生环境中的各种挑战。在本文中，我们强调了我们最近的努力在斑马鱼心电图采集具有成本效益的简化微电极阵列（MEA）膜提供多通道记录，一种新型的多室装置，同时筛选，和LabVIEW程序，以方便记录和处理。我们还展示了使用基于机器学习的程序来识别特定的ECG模式，利用我们目前有限的斑马鱼数据产生了有希望的结果。我们的解决方案有望开展大量心脏病研究、药物筛选、干细胞治疗验证和再生医学。
Heart disease is the leading cause of mortality in the U.S. with approximately 610,000 people dying every year. Effective therapies for many cardiac diseases are lacking, largely due to an incomplete understanding of their genetic basis and underlying molecular mechanisms. Zebrafish (Danio rerio) are an excellent model system for studying heart disease as they enable a forward genetic approach to tackle this unmet medical need. In recent years, our team has been employing electrocardiogram (ECG) as an efficient tool to study the zebrafish heart along with conventional approaches, such as immunohistochemistry, DNA and protein analyses. We have overcome various challenges in the small size and aquatic environment of zebrafish in order to obtain ECG signals with favorable signal-to-noise ratio (SNR), and high spatial and temporal resolution. In this paper, we highlight our recent efforts in zebrafish ECG acquisition with a cost-effective simplified microelectrode array (MEA) membrane providing multi-channel recording, a novel multi-chamber apparatus for simultaneous screening, and a LabVIEW program to facilitate recording and processing. We also demonstrate the use of machine learning-based programs to recognize specific ECG patterns, yielding promising results with our current limited amount of zebrafish data. Our solutions hold promise to carry out numerous studies of heart diseases, drug screening, stem cell-based therapy validation, and regenerative medicine.
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影响因子：
56.9
作者：
Poss, KD;Wilson, LG;Keating, MT
通讯作者：
Keating, MT
影响因子：
2.5
作者：
Cao, Hung;Yu, Fei;Hsiai, Tzung K.
通讯作者：
Hsiai, Tzung K.
影响因子：
4.6
作者：
Bournele, Despina;Beis, Dimitris
通讯作者：
Beis, Dimitris
DOI：
10.1152/ajpheart.00960.2005
发表时间：
2006-07-01
影响因子：
4.8
作者：
Milan, David J.;Jones, Ian L.;MacRae, Calum A.
通讯作者：
MacRae, Calum A.
影响因子：
2.9
作者：
Lin, Chia-Hung
通讯作者：
Lin, Chia-Hung