Photostable Ratiometric Pdot Probe for in Vitro and in Vivo Imaging of Hypochlorous Acid.
Photostable Ratiometric Pdot Probe for in Vitro and in Vivo Imaging of Hypochlorous Acid.
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DOI：
10.1021/jacs.7b01545
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发表时间：
2017-05-24
影响因子：
15
通讯作者：
Chiu DT
中科院分区：
文献类型：
--
作者：
Wu L;Wu IC;DuFort CC;Carlson MA;Wu X;Chen L;Kuo CT;Qin Y;Yu J;Hingorani SR;Chiu DT
活性氧 (ROS) 是许多病理生理过程的标志，开发用于检测活性氧 (ROS) 的探针对于了解 ROS 在许多危及生命的疾病中的精确作用和优化治疗干预措施至关重要。我们在此报告了一种基于一体式荧光半导体聚合物的远红至近红外（NIR）Pdot 纳米探针，用于次氯酸（HOCl）的比例检测。该制造利用灵活的聚合物设计的优势，将目标敏感和目标惰性荧光团结合到单个共轭聚合物中，以避免其他纳米探针中存在的泄漏或差异光漂白问题。所得纳米探针在HOCl传感方面具有改进的性能，如高亮度、理想的远红至近红外光学窗口、优异的光稳定性、自参考比率响应、快速响应和高选择性。双发射特性允许以高对比度对活体巨噬细胞中产生的 HOCl 波动和活体小鼠腹膜炎进行灵敏成像。这项研究不仅提供了一种强大且有前途的纳米探针，可用于研究生命系统中疾病的原位次氯酸状态，而且提出了一种新型比率荧光探针的设计策略，有助于在生物系统中进行精确可靠的测量。
Developing probes for the detection of reactive oxygen species (ROS), a hallmark of many pathophysiological process, is imperative to both understanding the precise roles of ROS in many life-threatening diseases and optimizing therapeutic interventions. We herein report an all-in-one fluorescent semiconducting polymer based far-red to near-infrared (NIR) Pdot nanoprobe for the ratiometric detection of hypochlorous acid (HOCl). The fabrication takes the advantage of flexible polymer design by incorporating target-sensitive and target-inert fluorophores into a single conjugated polymer to avoid leakage or differential photobleaching problems existed in other nanoprobes. The obtained nanoprobe has improved performance in HOCl sensing, such as high brightness, ideal far-red to NIR optical window, excellent photostability, self-referenced ratiometric response, fast response, and high selectivity. The dual-emission property allows the sensitive imaging of HOCl fluctuations produced in living macrophage cells and peritonitis of living mice with high contrast. This study not only provides a powerful and promising nanoprobe to be potentially used in the investigations of in situ HOCl status of diseases in living systems but also puts forward the design strategy of a new category of ratiometric fluorescent probes facilitating precise and reliable measurement in biological systems.
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