Urinary chemokines as noninvasive predictors of ulcerative interstitial cystitis.
Urinary chemokines as noninvasive predictors of ulcerative interstitial cystitis.
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DOI：
10.1016/j.juro.2012.01.034
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发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
Peters KM
中科院分区：
文献类型：
--
作者：
Tyagi P;Killinger K;Tyagi V;Nirmal J;Chancellor M;Peters KM
根据基础研究结果，趋化因子和细胞因子(CXCL-1和CXCL-10、神经生长因子和白介素6)的增加被认为是炎症和传入敏化的原因。在这项横断面研究中，我们检验了溃疡性和非溃疡性间质性膀胱炎/疼痛膀胱综合征患者尿液中选择性趋化因子增加的假设。收集10例溃疡性和非溃疡性间质性膀胱炎/疼痛综合征患者和10例无症状对照的中段尿标本。采用人细胞因子/趋化因子试验测定尿液中7种细胞因子水平。采用酶联免疫吸附试验检测神经生长因子。与溃疡性和非溃疡性间质性膀胱炎/疼痛膀胱综合征患者相比，无症状对照组的大多数趋化因子/细胞因子水平降低了10-100倍。对溃疡组和非溃疡组的8种测试蛋白质进行单变量比较，发现CXCL-10和CXCL-1、IL-6和神经生长因子(ANOVA p<；0.001)的表达显著增加了5到20倍。溃疡型和非溃疡型间质性膀胱炎/痛性膀胱综合征中趋化因子的差异表达提示了两种实体间旁分泌信号的差异。
Based on basic research findings an increase in chemokines and cytokines (CXCL-1 and 10, nerve growth factor and interleukin-6) is considered responsible for inflammation and afferent sensitization. In this cross-sectional study we tested the hypothesis that select chemokines are increased in the urine of patients with ulcerative and nonulcerative interstitial cystitis/painful bladder syndrome. Midstream urinary specimens were collected from 10 patients with ulcerative and nonulcerative interstitial cystitis/painful bladder syndrome, respectively, and from 10 asymptomatic controls. Urinary levels of 7 cytokines were measured by a human cytokine/chemokine assay. Nerve growth factor was measured by enzyme-linked immunosorbent assay. Urinary levels of most chemokines/cytokines were tenfold to 100-fold lower in asymptomatic controls vs patients with ulcerative and nonulcerative interstitial cystitis/painful bladder syndrome. Univariate comparison of 8 tested proteins in the ulcerative vs nonulcerative groups revealed a significant fivefold to twentyfold increase in CXCL-10 and 1, interleukin-6 and nerve growth factor (ANOVA p <0.001). Differential expression of chemokines in ulcerative and nonulcerative subtypes of interstitial cystitis/painful bladder syndrome suggests differences in paracrine signaling between the 2 entities.
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影响因子：
3.2
作者：
Antoniou, Katerina M.;Tzanakis, Nikolaos;Siafakas, Nikolaos M.
通讯作者：
Siafakas, Nikolaos M.
影响因子：
2.1
作者：
Peters, Kenneth M.;Killinger, Kim A.;Boura, Judith A.
通讯作者：
Boura, Judith A.
影响因子：
9.3
作者：
Kofoed, Kristian;Schneider, Uffe Vest;Eugen-Olsen, Jesper
通讯作者：
Eugen-Olsen, Jesper
影响因子：
6.6
作者：
Ogawa, Teruyuki;Homma, Toshiki;Nishizawa, Osamu
通讯作者：
Nishizawa, Osamu
DOI：
10.1186/1476-8518-6-6
发表时间：
2008-10-28
期刊：
Journal of immune based therapies and vaccines
影响因子：
--
作者：
Sakthivel, Senthilkumar K;Singh, Udai P;Lillard, James W Jr
通讯作者：
Lillard, James W Jr