Dental plaque development on a hydroxyapatite disk in young adults observed by using a barcoded pyrosequencing approach.
Dental plaque development on a hydroxyapatite disk in young adults observed by using a barcoded pyrosequencing approach.
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DOI：
10.1038/srep08136
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发表时间：
2015-01-30
影响因子：
4.6
通讯作者：
Yamashita Y
中科院分区：
文献类型：
--
作者：
Takeshita T;Yasui M;Shibata Y;Furuta M;Saeki Y;Eshima N;Yamashita Y
牙菌斑是一个动态的微生物生物膜生态系统，包括数百种难以培养的细菌。我们通过16S rRNA基因分析观察了菌斑细菌群落的组装。使用可移除树脂夹板从19名年轻成年人中收集牙菌斑样本，牙菌斑样本在第0天与唾液一起在羟基磷灰石盘上累积1、2、3、4、5和7天。定量PCR分析表明，细菌总量逐渐增加，并在第4天达到平台。条形码焦磷酸测序分析显示，微生物丰富度和多样性在第5天和第7天之间特别增加。基于未加权UniFrac的主坐标分析图显示了与时间相关的群落组装，其变得越来越类似于唾液微生物群。兼性厌氧菌，如链球菌，奈瑟氏球菌，Abiotrophia，Gemella和罗氏菌在早期的菌斑细菌群落中占主导地位，而专性厌氧菌，如卟啉单胞菌，梭杆菌，普雷沃氏菌和二氧化碳噬菌体在后期表现出更大的优势。UniFrac分析还表明，龋齿经历对组装过程有显著影响。我们的研究结果揭示了菌斑细菌群落的发展模式以及与龋齿经验相关的个体间差异。
Dental plaque is a dynamic microbial biofilm ecosystem that comprises hundreds of species including difficult-to-cultivate bacteria. We observed the assembly of a plaque bacterial community through 16S rRNA gene analysis. Plaque samples that accumulated on a hydroxyapatite disk for 1, 2, 3, 4, 5, and 7 days with saliva on day 0 were collected from 19 young adults using a removable resin splint. Quantitative PCR analysis showed that the total bacterial amount gradually increased and reached a plateau on day 4. Barcoded pyrosequencing analysis revealed that the microbial richness and diversity particularly increased between days 5 and 7. A principal coordinate analysis plot based on unweighted UniFrac showed the community assembly in a time-related manner, which became increasingly similar to the salivary microbiota. Facultative anaerobic bacteria such as Streptococcus, Neisseria, Abiotrophia, Gemella, and Rothia were predominant in the plaque bacterial community in the earlier days, whereas obligate anaerobes, such as Porphyromonas, Fusobacterium, Prevotella, and Capnocytophaga showed increased dominance on later days. UniFrac analysis also demonstrated that dental caries experience had a significant effect on the assembly process. Our results reveal the development pattern of the plaque bacterial community as well as the inter-individual differences associated with dental caries experience.
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影响因子：
10.7
作者：
Price MN;Dehal PS;Arkin AP
通讯作者：
Arkin AP
影响因子：
9.4
作者：
Tanner, A. C. R.;Mathney, J. M. J.;Dewhirst, F. E.
通讯作者：
Dewhirst, F. E.
影响因子：
--
作者：
Takeshita, T.;Nakano, Y.;Yamashita, Y.
通讯作者：
Yamashita, Y.
影响因子：
3
作者：
RITZ, HL
通讯作者：
RITZ, HL
影响因子：
4.4
作者：
Takeshita, Toru;Yasui, Masaki;Yamashita, Yoshihisa
通讯作者：
Yamashita, Yoshihisa