Gender-dependence of bone structure and properties in adult osteogenesis imperfecta murine model.
Gender-dependence of bone structure and properties in adult osteogenesis imperfecta murine model.
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DOI：
10.1007/s10439-013-0793-7
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发表时间：
2013-06
影响因子：
3.8
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Yao, Xiaomei;Carleton, Stephanie M.;Kettle, Arin D.;Melander, Jennifer;Phillips, Charlotte L.;Wang, Yong
成骨不全（OI）是一种以骨脆性和畸形为特征的显性骨骼疾病。尽管oim小鼠模型是OI模型中研究最广泛的，但直到最近才提出其在骨矿化方面表现出性别依赖性差异。为了表征性别对同种C57 BL/J6背景下oim骨的形态测量学/超微结构、机械性能和生化组成的影响，使用显微计算机断层扫描、三点弯曲和拉曼光谱评价了4个月大的oim/oim、+/oim和野生型（wt）雌性和雄性胫骨。在皮质骨和松质骨的形态和几何参数方面，性别差异明显。雄性小鼠天生比基因型匹配的雌性小鼠具有更多的骨骼和增加的惯性矩，骨骼生物力学强度也相应增加。性别的主要影响是骨生长和机械性能的结构/几何形状，而骨的矿物质/基质组成、羟脯氨酸含量主要受oim胶原突变的影响。这项研究提供了证据，性别的重要性，在评价和解释潜在的治疗策略时，使用小鼠模型的OI。
Osteogenesis imperfecta (OI) is a dominant skeletal disorder characterized by bone fragility and deformities. Though the oim mouse model has been the most widely studied of the OI models, it has only recently been suggested to exhibit gender-dependent differences in bone mineralization. To characterize the impact of gender on the morphometry/ultra-structure, mechanical properties, and biochemical composition of oim bone on the congenic C57BL/J6 background, 4-month-old oim/oim, +/oim, and wild-type (wt) female and male tibiae were evaluated using micro-computed tomography, three-point bending, and Raman spectroscopy. Dramatic gender differences were evident in both cortical and trabecular bone morphological and geometric parameters. Male mice had inherently more bone and increased moment of inertia than genotype-matched female counterparts with corresponding increases in bone biomechanical strength. The primary influence of gender was structure/geometry in bone growth and mechanical properties, whereas the mineral/matrix composition, hydroxyproline content of bone were influenced primarily by the oim collagen mutation. This study provides evidence of the importance of gender in the evaluation and interpretation of potential therapeutic strategies when using mouse models of OI.
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影响因子：
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