Detection of amyloid beta aggregates in the brain of BALB/c mice after Chlamydia pneumoniae infection.
Detection of amyloid beta aggregates in the brain of BALB/c mice after Chlamydia pneumoniae infection.
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肺炎衣原体感染后，检测BALB/C小鼠大脑中淀粉样蛋白β骨料。
DOI：
10.1007/s00401-007-0252-3
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发表时间：
2007-09
影响因子：
12.7
通讯作者：
Steinbusch, Harry W. M.
中科院分区：
文献类型：
--
作者：
Boelen, Ellen;Stassen, Frank R. M.;van der Ven, Andr J. A. M.;Lemmens, Marijke A. M.;Steinbusch, Hellen P. J.;Bruggeman, Cathrien A.;Schmitz, Christoph;Steinbusch, Harry W. M.
由脑感染引起的神经炎症越来越被认为是阿尔茨海默病（AD）等神经退行性疾病的病因。我们研究了肺炎衣原体（Cpn）感染是否会导致BALB/c小鼠细胞外β淀粉样蛋白（Aβ）聚集。在鼻内感染后1周，通过PCR主要在嗅球中检测到Cpn DNA，而在鼻内感染后1和3个月的大脑中检测到Cpn阴性。在1和3个月后，在Cpn感染小鼠的大脑中检测到细胞外Aβ免疫反应性，在模拟感染小鼠和未感染Cpn或模拟感染小鼠的大脑中也检测到。然而，与转基因APP751SL/PS1M146L小鼠大脑中检测到的细胞外Aβ聚集物相比，这些细胞外Aβ聚集物在形态上存在差异。这些数据并不像之前提出的那样明确支持Cpn感染诱导BALB/c小鼠大脑中ad样Aβ斑块形成的假设。然而，未来的研究需要解决这些差异，并调查Cpn是否确实是AD发病的一个病因因素。
Neuroinflammation, initiated by cerebral infection, is increasingly postulated as an aetiological factor in neurodegenerative diseases such as Alzheimer’s disease (AD). We investigated whether Chlamydia pneumoniae (Cpn) infection results in extracellular aggregation of amyloid beta (Aβ) in BALB/c mice. At 1 week post intranasal infection (p.i.), Cpn DNA was detected predominantly in the olfactory bulbs by PCR, whereas brains at 1 and 3 months p.i. were Cpn negative. At 1 and 3 months p.i., extracellular Aβ immunoreactivity was detected in the brain of Cpn-infected mice but also in the brain of mock-infected mice and mice that were neither Cpn infected nor mock infected. However, these extracellular Aβ aggregates showed morphological differences compared to extracellular Aβ aggregates detected in the brain of transgenic APP751SL/PS1M146L mice. These data do not unequivocally support the hypothesis that Cpn infection induces the formation of AD-like Aβ plaques in the brain of BALB/c mice, as suggested before. However, future studies are required to resolve these differences and to investigate whether Cpn is indeed an etiological factor in AD pathogenesis.
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