Defining key metabolic roles in osmotic adjustment and ROS homeostasis in the recretohalophyte Karelinia caspia under salt stress.
Defining key metabolic roles in osmotic adjustment and ROS homeostasis in the recretohalophyte Karelinia caspia under salt stress.
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DOI：
10.1111/ppl.13663
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发表时间：
2022-03
影响因子：
6.4
通讯作者：
Mur, Luis A. J.
中科院分区：
文献类型：
--
作者：
Guo, Qiang;Han, Jiwan;Li, Cui;Hou, Xincun;Zhao, Chunqiao;Wang, Qinghai;Wu, Juying;Mur, Luis A. J.
海草是一种具有牧草和药用价值的复盐植物，具有修复盐碱地的作用。本研究利用多组学方法评估了盐胁迫下白羊草（carelinia caspia）的初级/次级代谢对渗透调节和ROS稳态的贡献。计算机化表型评估、细胞渗透变化和脂质过氧化测试表明，盐处理对赤羊没有可检测到的物理影响。代谢组学分析表明，盐处理显著增加了氨基酸、糖类、有机酸、多胺、酚酸和维生素的积累。转录组学评估鉴定了盐胁迫下与上述初级/次级代谢物变化密切相关的差异表达基因。特别是，碳水化合物代谢（TCA循环，淀粉和蔗糖代谢，糖酵解）以及精氨酸和脯氨酸代谢的变化被观察到保持低渗透电位。绿原酸/维生素E的生物合成也得到了增强，这有助于在盐胁迫下荠菜清除活性氧。总的来说，我们的研究结果确定了主要/次要代谢的关键变化，这些变化协调调节渗透平衡和ROS稳态，从而促进了羊草的耐盐性。
The recretohalophyte Karelinia caspia is of forage and medical value and can remediate saline soils. We here assess the contribution of primary/secondary metabolism to osmotic adjustment and ROS homeostasis in Karelinia caspia under salt stress using multi‐omic approaches. Computerized phenomic assessments, tests for cellular osmotic changes and lipid peroxidation indicated that salt treatment had no detectable physical effect on K. caspia. Metabolomic analysis indicated that amino acids, saccharides, organic acids, polyamine, phenolic acids, and vitamins accumulated significantly with salt treatment. Transcriptomic assessment identified differentially expressed genes closely linked to the changes in above primary/secondary metabolites under salt stress. In particular, shifts in carbohydrate metabolism (TCA cycle, starch and sucrose metabolism, glycolysis) as well as arginine and proline metabolism were observed to maintain a low osmotic potential. Chlorogenic acid/vitamin E biosynthesis was also enhanced, which would aid in ROS scavenging in the response of K. caspia to salt. Overall, our findings define key changes in primary/secondary metabolism that are coordinated to modulate the osmotic balance and ROS homeostasis to contribute to the salt tolerance of K. caspia.
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