A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging.
A Labeling Strategy for Living Specimens in Long-Term/Super-Resolution Fluorescence Imaging.
复制标题
长期/超分辨率荧光成像中活体标本的标记策略
DOI：
10.3389/fchem.2020.601436
复制
发表时间：
2020
影响因子：
5.5
通讯作者：
Hao X
中科院分区：
文献类型：
--
作者：
Han Y;Zhang Z;Liu W;Yao Y;Xu Y;Liu X;Kuang C;Hao X
Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required to realize cell-permeability and specificity. In this study, we introduce a method to overcome these limits—taking advantage of the intrinsic affinity of bright and photostable fluorophores, no matter if they are supposed to be live-cell incompatible or not. Incubated with living cells and tissues in particular conditions (concentration and temperature), some Atto and BODIPY dyes show live-cell labeling capability for specific organelles without physical cell-penetration or chemical modifications. Notably, by using Atto 647N as a live-cell mitochondrial marker, we obtain 2.5-time enhancement of brightness and photostability compared with the most commonly used SiR dye in long-term imaging. Our strategy has expanded the scientist's toolbox for understanding the dynamics and interactions of subcellular structures in living specimens.
登录
查看更多内容
影响因子：
16.2
作者：
CORY, AH;OWEN, TC;CORY, JG
通讯作者：
CORY, JG
影响因子：
3.4
作者：
Bosch, Peter J.;Correa, Ivan R., Jr.;Subramaniam, Vinod
通讯作者：
Subramaniam, Vinod
影响因子：
10.8
作者：
Hennig, Simon;van de Linde, Sebastian;Sauer, Markus
通讯作者：
Sauer, Markus
影响因子：
46.9
作者：
Jaiswal, JK;Mattoussi, H;Simon, SM
通讯作者：
Simon, SM
使用 Hessian 结构照明显微镜进行快速、长期、超分辨率成像
影响因子：
46.9
作者：
Huang, Xiaoshuai;Fan, Junchao;Chen, Liangyi
通讯作者：
Chen, Liangyi