Ultra-localized single cell electroporation using silicon nanowires.
Ultra-localized single cell electroporation using silicon nanowires.
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DOI：
10.1039/c2lc40837f
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发表时间：
2013-02-07
期刊：
影响因子：
6.1
通讯作者：
Bashir R
中科院分区：
文献类型：
--
作者：
Jokilaakso N;Salm E;Chen A;Millet L;Guevara CD;Dorvel B;Reddy B Jr;Karlstrom AE;Chen Y;Ji H;Chen Y;Sooryakumar R;Bashir R
细胞间变异的分析可以进一步理解细胞内过程和单个细胞功能在较大细胞群体中的作用。精确裂解单细胞的能力可用于释放细胞组分，以解决在检查整个群体时可能被掩盖的细胞异质性。我们报道了一种在硅纳米线和纳米硅生物场效应晶体管上定位和裂解单个细胞的方法。在这项研究中，HT-29癌细胞通过使用外部磁场操纵磁珠被定位在晶体管的顶部。随后通过在10 MHz下施加600-900 mVpp在晶体管沟道和体衬底上进行短至2 ms的超快速细胞裂解。我们表明，在设备表面的边缘电场破坏细胞膜，导致不可逆的电穿孔裂解。该方法允许快速和简单的单细胞裂解和分析，在医学诊断、蛋白质组分析和发育生物学研究中具有潜在的应用。
Analysis of cell-to-cell variation can further the understanding of intracellular processes and the role of individual cell function within a larger cell population. The ability to precisely lyse single cells can be used to release cellular components to resolve cellular heterogeneity that might be obscured when whole populations are examined. We report a method to position and lyse individual cells on silicon nanowire and nanoribbon biological field effect transistors. In this study, HT-29 cancer cells were positioned on top of transistors by manipulating magnetic beads using external magnetic fields. Ultra-rapid cell lysis was subsequently performed by applying 600–900 mVpp at 10 MHz for as little as 2 ms across the transistor channel and the bulk substrate. We show that the fringing electric field at the device surface disrupts the cell membrane, leading to lysis from irreversible electroporation. This methodology allows rapid and simple single cell lysis and analysis with potential applications in medical diagnostics, proteome analysis and developmental biology studies.
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影响因子：
2.9
作者：
Lindstrom, Sara;Mori, Kiichiroh;Andersson-Svahn, Helene
通讯作者：
Andersson-Svahn, Helene
影响因子：
8.4
作者：
Hoefemann, Henning;Wadle, Simon;Zengerle, Roland
通讯作者：
Zengerle, Roland
影响因子：
3.7
作者：
Vieira, G.;Chen, A.;Sooryakumar, R.
通讯作者：
Sooryakumar, R.
影响因子：
6.1
作者：
Khine, M;Lau, A;Lee, LP
通讯作者：
Lee, LP
影响因子：
8.6
作者：
Vieira G;Henighan T;Chen A;Hauser AJ;Yang FY;Chalmers JJ;Sooryakumar R
通讯作者：
Sooryakumar R