Ail proteins of Yersinia pestis and Y. pseudotuberculosis have different cell binding and invasion activities.
Ail proteins of Yersinia pestis and Y. pseudotuberculosis have different cell binding and invasion activities.
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DOI：
10.1371/journal.pone.0083621
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Krukonis ES
中科院分区：
文献类型：
--
作者：
Tsang TM;Wiese JS;Felek S;Kronshage M;Krukonis ES
鼠疫耶尔森氏菌粘附素Ail介导宿主细胞结合并促进细胞毒性Yop蛋白的递送。从Y。鼠疫菌和Y.假结核除了在位置43和126处的一个或两个氨基酸（取决于Y）之外是相同的。假结核菌株从Y。已经报道假结核菌株YPIII缺乏宿主细胞结合能力，因此我们试图确定哪种氨基酸差异是细胞粘附差异的原因。Y. pseudotuberculosis YPIII Ail在大肠杆菌结合宿主细胞中表达，但表达量仅为Y.鼠疫Y.鼠疫杆菌Ail单突变体Ail-E43 D和Ail-F126 V在E.大肠杆菌中，与野生型Y.鼠疫Y.假结核YPIII Ail与Ail底物纤连蛋白的结合也相对于Y.鼠疫菌E. pestis Ail.杆菌当用Y表示时。鼠疫杆菌，粘附和侵袭降低30-50%，这取决于取代。Ail介导的Yop通过Y.鼠疫菌Ail和鼠疫杆菌Y. pseudotuberculosis Ail在Y.鼠疫，仅Ail-F126 V在统计学上显著降低Yop递送25%。与E. coli和Y. pestis中Ail的表达。假结核导致不可测量的粘附或侵袭，提示Y.假结核干扰Ail细胞结合活性。因此，宿主环境影响Ail和Y.鼠疫菌和Y.假结核Ail可以介导细胞结合、细胞侵袭并促进Yop递送。
The Yersinia pestis adhesin Ail mediates host cell binding and facilitates delivery of cytotoxic Yop proteins. Ail from Y. pestis and Y. pseudotuberculosis is identical except for one or two amino acids at positions 43 and 126 depending on the Y. pseudotuberculosis strain. Ail from Y. pseudotuberculosis strain YPIII has been reported to lack host cell binding ability, thus we sought to determine which amino acid difference(s) are responsible for the difference in cell adhesion. Y. pseudotuberculosis YPIII Ail expressed in Escherichia coli bound host cells, albeit at ∼50% the capacity of Y. pestis Ail. Y. pestis Ail single mutants, Ail-E43D and Ail-F126V, both have decreased adhesion and invasion in E. coli when compared to wild-type Y. pestis Ail. Y. pseudotuberculosis YPIII Ail also had decreased binding to the Ail substrate fibronectin, relative to Y. pestis Ail in E. coli. When expressed in Y. pestis, there was a 30–50% decrease in adhesion and invasion depending on the substitution. Ail-mediated Yop delivery by both Y. pestis Ail and Y. pseudotuberculosis Ail were similar when expressed in Y. pestis, with only Ail-F126V giving a statistically significant reduction in Yop delivery of 25%. In contrast to results in E. coli and Y. pestis, expression of Ail in Y. pseudotuberculosis led to no measurable adhesion or invasion, suggesting the longer LPS of Y. pseudotuberculosis interferes with Ail cell-binding activity. Thus, host context affects the binding activities of Ail and both Y. pestis and Y. pseudotuberculosis Ail can mediate cell binding, cell invasion and facilitate Yop delivery.
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DOI：
10.1073/pnas.89.8.3561
发表时间：
1992-04-15
影响因子：
11.1
作者：
BLISKA, JB;FALKOW, S
通讯作者：
FALKOW, S
影响因子：
3.6
作者：
BLOMFIELD, IC;MCCLAIN, MS;EISENSTEIN, BI
通讯作者：
EISENSTEIN, BI
影响因子：
3.1
作者：
Felek, Suleyman;Krukonis, Eric S.
通讯作者：
Krukonis, Eric S.
影响因子：
6.6
作者：
Boyd, AP;Grosdent, N;Cornelis, GR
通讯作者：
Cornelis, GR
影响因子：
2.1
作者：
HEESEMANN, J;GRUTER, L
通讯作者：
GRUTER, L