Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis.
Tapetal 3-Ketoacyl-Coenzyme A Synthases Are Involved in Pollen Coat Lipid Accumulation for Pollen-Stigma Interaction in Arabidopsis.
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绒毡层 3-酮脂酰辅酶 A 合成酶参与拟南芥花粉-柱头相互作用的花粉皮脂质积累
DOI：
10.3389/fpls.2021.770311
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发表时间：
2021
影响因子：
5.6
通讯作者：
Yang ZN
中科院分区：
文献类型：
--
作者：
Zhang Z;Zhan H;Lu J;Xiong S;Yang N;Yuan H;Yang ZN
花粉被脂质是保护花粉的外部屏障，在花粉与柱头的相互作用中起着重要作用。然而，在花药发育过程中，其产生、沉积、调节和功能的分子机制仍然很模糊。在脂质代谢中，3-酮脂酰辅酶A脱氢酶（KCS）参与脂肪酸延长或极长链脂肪酸（VLCFA）合成。在这项研究中，我们确定了六个成员的拟南芥KCS家族在花药中表达。其中，KCS 7、KCS 15和KCS 21在花药8-10期的绒毡层细胞中表达。进一步的分析表明，他们的下游雄性不育1（MS 1），后期绒毡层发育的调节器。kcs 7/15/21三重突变体是可育的。细胞学观察和脂质染色均表明，突变体kcs 7/15/21的花粉囊脂含量降低。落在柱头上后，野生型花粉粒水合约5分钟，而kcs 7/15/21三重突变花粉水合约10分钟。三突变体的花粉管生长也被延迟。这些结果表明，绒毡层定位的KCS蛋白参与的积累的花粉外壳脂质和揭示绒毡层来源的花粉外壳脂质的花粉-柱头相互作用的作用。
Pollen coat lipids form an outer barrier to protect pollen itself and play essential roles in pollen-stigma interaction. However, the precise molecular mechanisms underlying the production, deposition, regulation, and function of pollen coat lipids during anther development remain largely elusive. In lipid metabolism, 3-ketoacyl-coenzyme A synthases (KCS) are involved in fatty acid elongation or very-long-chain fatty acid (VLCFA) synthesis. In this study, we identified six members of the Arabidopsis KCS family expressed in anther. Among them, KCS7, KCS15, and KCS21 were expressed in tapetal cells at anther stages 8–10. Further analysis demonstrated that they act downstream of male sterility 1 (MS1), a regulator of late tapetum development. The kcs7/15/21 triple mutant is fertile. Both cellular observation and lipid staining showed pollen coat lipid was decreased in kcs7/15/21 triple mutant. After landing on stigma, the wild-type pollen grains were hydrated for about 5 min while the kcs7/15/21 triple mutant pollen took about 10 min to hydrate. Pollen tube growth of the triple mutant was also delayed. These results demonstrate that the tapetum-localized KCS proteins are involved in the accumulation of pollen coat lipid and reveal the roles of tapetal-derived pollen coat lipid for pollen-stigma interaction.
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影响因子：
11.6
作者：
Fiebig, A;Mayfield, JA;Preuss, D
通讯作者：
Preuss, D
影响因子：
7.4
作者：
Alves-Ferreira, Marcio;Wellmer, Frank;Meyerowitz, Elliot M.
通讯作者：
Meyerowitz, Elliot M.
DOI：
10.1007/978-3-319-25979-6_1
发表时间：
2016-01-01
期刊：
LIPIDS IN PLANT AND ALGAE DEVELOPMENT
影响因子：
--
作者：
Li-Beisson, Yonghua;Nakamura, Yuki;Harwood, John
通讯作者：
Harwood, John
影响因子：
7.4
作者：
Hooker, TS;Millar, AA;Kunst, L
通讯作者：
Kunst, L
影响因子：
21.3
作者：
Mayfield, JA;Preuss, D
通讯作者：
Preuss, D