Detailed structural analysis of lipids directly on tissue specimens using a MALDI-SpiralTOF-Reflectron TOF mass spectrometer.

Detailed structural analysis of lipids directly on tissue specimens using a MALDI-SpiralTOF-Reflectron TOF mass spectrometer.
复制标题

DOI:
10.1371/journal.pone.0037107
复制
发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Toyoda M
Toyoda M
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Shimma S;Kubo A;Satoh T;Toyoda M

文献摘要

参考文献

被引文献

相似文献

描述了使用新型串联飞行时间(TOF-TOF)质谱仪的直接组织分析。该系统由基质辅助激光解吸/电离离子源、螺旋离子轨迹TOF质谱仪“SpiralTOF(STOF)"、碰撞池和偏置抛物反射器(RTOF)组成。该系统的特点是高前体离子的选择性,由于17米的飞行路径长度在STOF和消除后源衰变(PSD)离子。加速能量为20 keV,因此高能碰撞诱导解离(HE-CID)是可能的。PSD离子的消除允许观察HE-CID过程固有的产物离子。通过使用该串联TOF仪器,脂质的产物离子谱提供了脂肪酸残基的详细结构信息。
Direct tissue analysis using a novel tandem time-of-flight (TOF-TOF) mass spectrometer is described. This system consists of a matrix-assisted laser desorption/ionization ion source, a spiral ion trajectory TOF mass spectrometer “SpiralTOF (STOF)”, a collision cell, and an offset parabolic reflectron (RTOF). The features of this system are high precursor ion selectivity due to a 17-m flight path length in STOF and elimination of post-source decay (PSD) ions. The acceleration energy is 20 keV, so that high-energy collision-induced dissociation (HE-CID) is possible. Elimination of PSD ions allows observation of the product ions inherent to the HE-CID process. By using this tandem TOF instrument, the product ion spectrum of lipids provided detailed structural information of fatty acid residues.
DOI: 10.1007/s00216-010-3723-7
发表时间: 2010-08
影响因子: 4.3
作者:
Chen R;Li L
通讯作者: Li L
DOI: 10.1002/rcm.4939
发表时间: 2011-04-15
影响因子: 2
作者:
Goodwin, Richard J. A.;Pitt, Andrew R.;Harrison, David;Weidt, Stefan K.;Langridge-Smith, Pat R. R.;Barrett, Michael P.;Mackay, C. Logan
通讯作者: Mackay, C. Logan
DOI: 10.1007/s00216-011-4802-0
发表时间: 2011-07-01
影响因子: 4.3
作者:
Marsching, Christian;Eckhardt, Matthias;Hopf, Carsten
通讯作者: Hopf, Carsten
DOI: 10.1007/978-1-60761-746-4_12
发表时间: 2010-01-01
期刊: MASS SPECTROMETRY IMAGING: PRINCIPLES AND PROTOCOLS
影响因子: --
作者:
Garrett, Timothy J.;Yost, Richard A.
通讯作者: Yost, Richard A.
DOI: 10.1002/rcm.2486
发表时间: 2006-01-01
影响因子: 2
作者:
Belgacem, Omar;Bowdler, Andrew;Raptakis, Emmanuel
通讯作者: Raptakis, Emmanuel