Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole.
Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole.
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DOI：
10.1083/jcb.200105056
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发表时间：
2001-08-06
期刊：
影响因子：
--
通讯作者：
St Johnston D
中科院分区：
文献类型：
--
作者：
van Eeden FJ;Palacios IM;Petronczki M;Weston MJ;St Johnston D
奥斯卡在果蝇卵母细胞后极的定位诱导了极质的组装，因此确定了胚胎中腹部和生殖细胞形成的位置。这种定位是通过将oskar mRNA靶向后部并本地化激活其翻译来实现的。oskar mRNA似乎可能沿着微管主动运输，因为它的定位需要一个完整的微管细胞骨架和正端定向马达驱动蛋白I，但关于RNA如何与马达偶联尚不清楚。在这里，我们描述了barentsz，一个新的基因需要定位奥斯卡mRNA。与所有其他破坏这一过程的突变相反，barentsz-null突变完全阻断了oskar mRNA的后定位，而不影响bicoid和gurken mRNA的定位、微管的组织或极质组装的后续步骤。令人惊讶的是，大多数突变胚胎仍然形成腹部，这表明oskar mRNA定位与翻译控制部分冗余。Barentsz蛋白与oskar mRNA共定位于后部，这种定位依赖于oskar mRNA。因此，Barentsz对于oskar mRNA的后定位至关重要，并作为oskar RNA转运复合物的特定组分。
The localization of Oskar at the posterior pole of the Drosophila oocyte induces the assembly of the pole plasm and therefore defines where the abdomen and germ cells form in the embryo. This localization is achieved by the targeting of oskar mRNA to the posterior and the localized activation of its translation. oskar mRNA seems likely to be actively transported along microtubules, since its localization requires both an intact microtubule cytoskeleton and the plus end–directed motor kinesin I, but nothing is known about how the RNA is coupled to the motor. Here, we describe barentsz, a novel gene required for the localization of oskar mRNA. In contrast to all other mutations that disrupt this process, barentsz-null mutants completely block the posterior localization of oskar mRNA without affecting bicoid and gurken mRNA localization, the organization of the microtubules, or subsequent steps in pole plasm assembly. Surprisingly, most mutant embryos still form an abdomen, indicating that oskar mRNA localization is partially redundant with the translational control. Barentsz protein colocalizes to the posterior with oskar mRNA, and this localization is oskar mRNA dependent. Thus, Barentsz is essential for the posterior localization of oskar mRNA and behaves as a specific component of the oskar RNA transport complex.
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