Post-metamorphic skeletal growth in the sea urchin Paracentrotus lividus and implications for body plan evolution.

Post-metamorphic skeletal growth in the sea urchin Paracentrotus lividus and implications for body plan evolution.
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DOI:
10.1186/s13227-021-00174-1
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发表时间:
2021-03-16
期刊:
影响因子:
4.1
通讯作者:
Oliveri P
Oliveri P
中科院分区:
生物学2区
文献类型:
--
作者:
Thompson JR;Paganos P;Benvenuto G;Arnone MI;Oliveri P

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了解支撑动物发育的分子和细胞过程对于理解当今地球上发现的身体结构的多样性至关重要。由于它们在化石记录中的丰富,以及在实验室中作为模型系统的可追溯性,骨骼为理解动物多样性的起源提供了理想的实验模型。本文利用分子和细胞标记来了解海胆幼体骨骼的生长发育情况。我们根据正常棘球蚴的变质后第1 ~ 4周的生命制定了详细的分期方案。我们将该方案与神经元、肌肉和骨骼组织的免疫组织化学染色以及骨骼生长和细胞增殖的荧光测定相结合,以了解海胆体计划骨骼生长和发育的分子和细胞机制。我们的实验强调了骨骼生成蛋白在骨骼增生生长和细胞增殖中的作用。此外,本研究为理解海胆体计划在宏观进化时间尺度上的发育演化提供了一个框架。在线版本包含补充材料,可在10.1186/s13227-021-00174-1获得。
Understanding the molecular and cellular processes that underpin animal development are crucial for understanding the diversity of body plans found on the planet today. Because of their abundance in the fossil record, and tractability as a model system in the lab, skeletons provide an ideal experimental model to understand the origins of animal diversity. We herein use molecular and cellular markers to understand the growth and development of the juvenile sea urchin (echinoid) skeleton. We developed a detailed staging scheme based off of the first ~ 4 weeks of post-metamorphic life of the regular echinoid Paracentrotus lividus. We paired this scheme with immunohistochemical staining for neuronal, muscular, and skeletal tissues, and fluorescent assays of skeletal growth and cell proliferation to understand the molecular and cellular mechanisms underlying skeletal growth and development of the sea urchin body plan. Our experiments highlight the role of skeletogenic proteins in accretionary skeletal growth and cell proliferation in the addition of new metameric tissues. Furthermore, this work provides a framework for understanding the developmental evolution of sea urchin body plans on macroevolutionary timescales. The online version contains supplementary material available at 10.1186/s13227-021-00174-1.
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