Multimodality microscopy of cell dynamics in three-dimensional engineered and natural tissues
Multimodality microscopy of cell dynamics in three-dimensional engineered and natural tissues
复制标题
三维工程组织和天然组织中细胞动力学的多模态显微镜
DOI：
10.1109/iccme.2009.4906690
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
S. Boppart
中科院分区：
文献类型：
--
作者：
S. Boppart
我们对人类疾病的理解取决于我们可视化和跟踪组织中发生的动态分子和细胞变化的能力。光学显微镜技术为复杂的三维（3-D）组织微环境的非破坏性研究提供了适当的时空尺度。在组织工程中，人们已经认识到，与标准平面基底相比，3D 支架更能准确地再现自然微环境。同样，我们认识到机械刺激对细胞动态行为和复杂组织形成的重要性。虽然 3-D 支架受到青睐，但我们用于可视化这些 3-D 结构的显微成像技术却受到限制。然而，显微镜技术的最新进展，包括光学相干和多光子显微镜，使得高分辨率的深层组织成像成为可能，甚至在高度散射的工程组织和天然组织中也是如此。使用新型多模态显微镜平台，我们对一系列工程组织中的单细胞和小细胞群的动态进行了成像，并在人体皮肤中展示了体内成像。还观察到机械刺激的影响，使未来的发展能够通过工程组织的生长、在机械刺激下以及移植到活宿主中来跟踪细胞群。随着干细胞生物学的进步，标记和跟踪也成为可能，有助于阐明干细胞在其微环境中的迁移和归巢。预计这些先进的显微镜技术将揭示新的见解，并为干细胞生物学、复杂系统生物学、疾病发病机制以及体内应用的工程组织的优化提供实验数据。
Our understanding of human disease is dependent on our ability to visualize and track the dynamic molecular and cellular changes that occur in tissue. Optical microscopy techniques provided the appropriate spatiotemporal scales for the non-destructive investigation into the complex three-dimensional (3-D) microenvironment of tissue. In tissue engineering, it has been recognized that 3-D scaffolds more closely recapitulate the natural microenvironment compared to standard planar substrates. Similarly, we are recognizing the importance that mechanical stimuli have on the dynamic behavior of cells and the formation of complex tissues. While 3-D scaffolds are favored, our microscopic imaging technologies to visualize these 3-D structures have been limited. Recent advances in microscopy, however, including optical coherence and multi-photon microscopy, have made high-resolution, deep-tissue imaging possible, even in highly-scattering engineered and natural tissues. Using a novel multimodal microscopy platform, we have imaged the dynamics of single cells and small populations of cells in a range of engineered tissues, as well as demonstrated in vivo imaging in human skin. Effects from mechanical stimulation are also observed, enabling future developments to track populations of cells through the growth of engineered tissues, under the application of mechanical stimuli, and following the grafting into living hosts. With advances in stem cell biology, labeling and tracking are also possible, helping to elucidate the migration and homing of stem cells to and from their niches. It is expected that these advanced microscopy techniques will reveal new insight and contribute experimental data to work in stem cell biology, complex systems biology, disease pathogenesis, and the optimization of engineered tissues for in vivo applications.
影响因子：
3.6
作者：
Aguirre, AD;Hsiung, P;Fujimoto, JG
通讯作者：
Fujimoto, JG
影响因子：
3.5
作者：
Tang, Shuo;Krasieva, Tatiana B.;Tromberg, Bruce J.
通讯作者：
Tromberg, Bruce J.