Distinct spatiotemporal activity in principal neurons of the mouse olfactory bulb in anesthetized and awake states.
Distinct spatiotemporal activity in principal neurons of the mouse olfactory bulb in anesthetized and awake states.
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DOI：
10.3389/fncir.2013.00046
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发表时间：
2013
影响因子：
3.5
通讯作者：
Murthy VN
中科院分区：
文献类型：
--
作者：
Blauvelt DG;Sato TF;Wienisch M;Knöpfel T;Murthy VN
哺乳动物嗅觉信息的获得和早期加工是通过嗅闻行为和神经反馈来调节的。我们成像的时空模式的气味诱发的活动在一个人口的输出神经元（二尖瓣/簇状细胞，MTCs）在头部限制小鼠的嗅球（OB）表达基因编码的钙指标。在麻醉动物中，MTC群体活动的时间动态相对简单，但在清醒动物中变化很大。然而，清醒动物的明显不规则的活动可以很好地预测使用外部测量的嗅定时，或通过MTC群体的全局响应的波动来推断，即使没有明确的嗅时间的知识。整个空间模式的活动是保守的状态，但气味反应有一个弥漫性的空间组成部分，在清醒状态下不太突出的麻醉小鼠。多光子显微镜表明，MTC侧树突的可能来源的空间分散的反应在麻醉动物。我们的数据表明，MTCs的时间和空间动态可以显着调制的行为状态，和MTCs的合奏活动可以提供有关嗅定时下游电路的信息，以帮助解码气味反应。
The acquisition of olfactory information and its early processing in mammals are modulated by brain states through sniffing behavior and neural feedback. We imaged the spatiotemporal pattern of odor-evoked activity in a population of output neurons (mitral/tufted cells, MTCs) in the olfactory bulb (OB) of head-restrained mice expressing a genetically-encoded calcium indicator. The temporal dynamics of MTC population activity were relatively simple in anesthetized animals, but were highly variable in awake animals. However, the apparently irregular activity in awake animals could be predicted well using sniff timing measured externally, or inferred through fluctuations in the global responses of MTC population even without explicit knowledge of sniff times. The overall spatial pattern of activity was conserved across states, but odor responses had a diffuse spatial component in anesthetized mice that was less prominent during wakefulness. Multi-photon microscopy indicated that MTC lateral dendrites were the likely source of spatially disperse responses in the anesthetized animal. Our data demonstrate that the temporal and spatial dynamics of MTCs can be significantly modulated by behavioral state, and that the ensemble activity of MTCs can provide information about sniff timing to downstream circuits to help decode odor responses.
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