Time-lapse mechanical imaging of neural tube closure in live embryo using Brillouin microscopy.
Time-lapse mechanical imaging of neural tube closure in live embryo using Brillouin microscopy.
复制标题
使用Brillouin显微镜在活胚中神经管闭合的延时机械成像。
DOI：
10.1038/s41598-023-27456-z
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发表时间：
2023-01-06
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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作者：
神经管闭合（NTC）是一个复杂的胚胎发育过程，涉及分子、细胞和生物力学机制。虽然遗传因素和生化信号已被广泛研究，但由于缺乏工具，组织生物力学的作用仍未得到充分研究。在这里，我们开发了一种光学模式，可以在胚胎经历神经发育时对神经板组织进行延时机械成像。该技术是基于共聚焦布里渊显微镜和一种改良的鸡胚体外培养与舞台孵化器的结合。通过这项技术，我们第一次用活胚胎捕捉到了神经板组织的机械进化过程。具体来说，我们观察到体外培养的胚胎在NTC过程中神经板的组织模量持续增加，这与卵内培养的数据以及以往的研究结果一致。除此之外，我们发现组织模量的增加与组织增厚和弯曲高度相关。我们预见这种非接触和无标签的技术为理解胚胎发育的生物力学机制开辟了新的机会。
Neural tube closure (NTC) is a complex process of embryonic development involving molecular, cellular, and biomechanical mechanisms. While the genetic factors and biochemical signaling have been extensively investigated, the role of tissue biomechanics remains mostly unexplored due to the lack of tools. Here, we developed an optical modality that can conduct time-lapse mechanical imaging of neural plate tissue as the embryo is experiencing neurulation. This technique is based on the combination of a confocal Brillouin microscope and a modified ex ovo culturing of chick embryo with an on-stage incubator. With this technique, for the first time, we captured the mechanical evolution of the neural plate tissue with live embryos. Specifically, we observed the continuous increase in tissue modulus of the neural plate during NTC for ex ovo cultured embryos, which is consistent with the data of in ovo culture as well as previous studies. Beyond that, we found that the increase in tissue modulus was highly correlated with the tissue thickening and bending. We foresee this non-contact and label-free technique opening new opportunities to understand the biomechanical mechanisms in embryonic development.
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影响因子：
64.8
作者：
Barriga EH;Franze K;Charras G;Mayor R
通讯作者：
Mayor R
DOI：
10.1073/pnas.1700934114
发表时间：
2017-06-27
影响因子：
11.1
作者：
Galea, Gabriel L.;Cho, Young-June;Copp, Andrew J.
通讯作者：
Copp, Andrew J.
影响因子：
2.4
作者：
Davidson, Lance A.;Joshi, Sagar D.;Zhou, Jian
通讯作者：
Zhou, Jian
影响因子：
3.5
作者：
Raghunathan, Raksha;Zhang, Jitao;Scarcelli, Giuliano
通讯作者：
Scarcelli, Giuliano
DOI：
10.1038/nrg3513
发表时间：
2013-10
期刊：
Nature reviews. Genetics
影响因子：
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作者：
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