Calmodulin concentrates at the apex of growing hyphae and localizes to the Spitzenkörper in Aspergillus nidulans

Calmodulin concentrates at the apex of growing hyphae and localizes to the Spitzenkörper in Aspergillus nidulans
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钙调蛋白集中在生长菌丝的顶端,并定位于构巢曲霉的 Spitzenkörper

DOI:
10.1007/s00709-006-0181-3
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发表时间:
2006-09
期刊:
影响因子:
2.9
通讯作者:
张超英
张超英
中科院分区:
生物学3区
文献类型:
--
作者:
陆玲;A. Singapuri;袁生;王刚;张超英

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摘要:利用功能性 GFP::CaM 融合蛋白研究了构巢曲霉生长的菌丝尖端中钙调蛋白 (CaM) 的定位模式。在生长的菌丝尖端中发现了微弱的尖端高梯度,CaM 高度集中在与形成明亮颗粒的 Spitzenkörper 相对应的区域中。最顶端高度浓缩的 CaM 的位置似乎决定了菌丝的方向。 CaM 定位的正常模式也被证明依赖于整合的肌动蛋白细胞骨架。当菌丝尖端停止生长时,CaM未能定位在亮颗粒中,而是均匀分布在菌丝尖端。这些发现表明,CaM 可能在构巢曲霉的建立和维持顶端组织、形态发生和生长中发挥重要作用。
Summary.The calmodulin (CaM) localization pattern in the growing hyphal tip of Aspergillus nidulans was studied with the functional GFP::CaM fusion protein. A faint tip-high gradient of CaM was found in the growing hyphal tip, with CaM highly localized in the region corresponding to the Spitzenkörper forming a bright granule. The position of highly concentrated CaM in the extreme apex seemed to determine the orientation of the hypha. The normal pattern of CaM localization was also shown to be dependent on the integrated actin cytoskeleton. When the growth of the hyphal tip ceased, CaM failed to localize in the bright granule and was evenly distributed in the hyphal tip. These findings suggest that CaM may play an important role in establishing and maintaining apical organization, morphogenesis, and growth in Aspergillus nidulans.
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发表时间: 1997
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