Influence of testosterone on phase II metabolism and availability of soy isoflavones in male Wistar rats
Influence of testosterone on phase II metabolism and availability of soy isoflavones in male Wistar rats
复制标题
睾酮对雄性 Wistar 大鼠 II 期代谢和大豆异黄酮有效性的影响
DOI：
10.1007/s00204-016-1853-1
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发表时间：
2016
影响因子：
6.1
通讯作者：
Kulling SE
中科院分区：
文献类型：
--
作者：
Soukup ST;Müller DR;Kurrat A;Diel P;Kulling SE
染料木黄酮和大豆黄酮是大豆中主要的异黄酮。它们对男性的潜在有利或不利影响，如预防前列腺癌或对生殖功能的影响，在讨论中存在争议。其生物活性的主要决定因素是异黄酮的吸收和生物转化。在本研究中，我们关注睾酮对血浆可利用性和异黄酮II期代谢的影响。雄性Wistar大鼠给予富含异黄酮的饮食，随机分为三组：两组在出生后第80天（PND）切除睾丸（ORX），在持续7天的激素下降后给予丙酸睾酮（TP） （ORX TP组）或对照物（ORX组）治疗11天。第三组作为对照组，保持完整。大鼠于PND 98处死。ORX大鼠血浆异黄酮水平降低。与肝脏和肾脏II期代谢物运输相关的转运蛋白mRNA表达的不同调控可能是导致这种减少的原因，更准确地说，肾脏中的Slc10a1和Slc21a1以及肝脏中的Slc22a8。虽然完整大鼠的主要II期代谢物是硫酸酯和磺脲嘧啶，但ORX显著减少了硫酸酯偶联物的数量。由此可见，ORX可降低肝脏中不同硫转移酶mRNA的表达。经TP处理后，所观察到的效果几乎可以恢复。综上所述，睾酮以及其他可能的雄激素通过影响不同硫转移酶和转运蛋白的表达，对异黄酮的II期代谢和可利用性产生巨大影响。
Genistein and daidzein are the main isoflavones in soy. Their potential beneficial or adverse effects in males like the prevention of prostate cancer or the impact on reproductive functions are controversially discussed. Major determinants of their bioactivity are the absorption and biotransformation of isoflavones. In this study, we focused on the influence of testosterone on plasma availability and phase II metabolism of isoflavones. Male Wistar rats, receiving an isoflavones rich diet, were randomized into three groups: Two groups were orchiectomized (ORX) at postnatal day (PND) 80 and treated for 11 days with testosterone propionate (TP) (ORX TP group) or a vehicle (ORX group) after a 7 days lasting hormonal decline. The third group served as control and remained intact. Rats were sacrificed at PND 98. ORX rats had reduced isoflavones plasma levels. Differently regulated mRNA expressions of transporters relevant for transport of phase II metabolites in liver and kidney may be responsible for this reduction, more precisely Slc10a1 and Slc21a1 in kidney as well as Slc22a8 in liver. While main phase II metabolites in intact rats were disulfates and sulfoglucuronides, the amount of sulfate conjugates was significantly diminished by ORX. In accordance with that, mRNA expression of different sulfotransferases was reduced in liver by ORX. The observed effects could be almost restored by TP treatment. In conclusion, testosterone, and likely further androgens, has a huge impact on phase II metabolism and availability of isoflavones by influencing the expression of different sulfotransferases and transporters.
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影响因子：
3.9
作者：
Shelby, MK;Cherrington, NJ;Klaassen, CD
通讯作者：
Klaassen, CD
DOI：
10.1124/jpet.301.1.145
发表时间：
2002-04-01
影响因子：
3.5
作者：
Buist, SCN;Cherrington, NJ;Klaassen, CD
通讯作者：
Klaassen, CD
影响因子：
4.2
作者：
Sfakianos, J;Coward, L;Barnes, S
通讯作者：
Barnes, S
DOI：
--
发表时间：
1998-06
期刊：
Drug metabolism and disposition: the biological fate of chemicals
影响因子：
--
作者：
Robert T. Dunn;Curtis D. Klaassen
通讯作者：
Robert T. Dunn;Curtis D. Klaassen
成年雄性大鼠去势后血浆雄激素水平随时间的生理变化
DOI：
--
发表时间：
1988
期刊：
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
影响因子：
--
作者：
S. Andò;S. Aquila;E. Beraldi;M. Canonaco;M. L. Panno;A. Valenti;F. Dessì
通讯作者：
F. Dessì