Simian varicella virus infection of Chinese rhesus macaques produces ganglionic infection in the absence of rash.
Simian varicella virus infection of Chinese rhesus macaques produces ganglionic infection in the absence of rash.
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DOI：
10.1007/s13365-012-0083-4
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发表时间：
2012-04
影响因子：
3.2
通讯作者：
Verjans, Georges M. G. M.
中科院分区：
文献类型：
--
作者：
Ouwendijk, Werner J. D.;Mahalingam, Ravi;Traina-Dorge, Vicki;van Amerongen, Geert;Wellish, Mary;Osterhaus, Albert D. M. E.;Gilden, Don;Verjans, Georges M. G. M.
水痘-带状疱疹病毒（VZV）引起水痘，潜伏在神经节沿着整个轴突，并可能重新激活引起带状疱疹。VZV可通过从感染皮肤的逆行轴突运输或通过血行传播感染神经节。猴水痘病毒（SVV）感染恒河猴为研究人类VZV感染的发病机制提供了一个有用的模型系统。为了研究SVV急性感染过程中病毒和宿主免疫因素的变化，我们分析了4只SVV阴性的中国恒河猴，它们分别用5 × 103 pfu的表达SVV的绿色荧光蛋白（n = 2）和5 × 104 pfu的野生型SVV（n = 2）进行肠道感染。所有猴子都出现病毒血症和SVV特异性适应性B和T细胞免疫应答，但没有出现皮疹。在感染后21天的尸检中，在沿着整个神经轴的神经节和内脏中发现SVV DNA，并且在神经节中发现SVV RNA，但不在内脏中。SVV接种量与病毒血症的程度和对病毒的免疫应答有关。我们的研究结果表明，急性SVV感染中国恒河猴导致神经节感染的血源性途径和诱导病毒特异性适应性记忆反应的情况下皮疹。
Varicella-zoster virus (VZV) causes varicella (chickenpox), becomes latent in ganglia along the entire neuraxis, and may reactivate to cause herpes zoster (shingles). VZV may infect ganglia via retrograde axonal transport from infected skin or through hematogenous spread. Simian varicella virus (SVV) infection of rhesus macaques provides a useful model system to study the pathogenesis of human VZV infection. To dissect the virus and host immune factors during acute SVV infection, we analyzed four SVV-seronegative Chinese rhesus macaques infected intratracheally with cell-associated 5 × 103 plaque-forming units (pfu) of SVV-expressing green fluorescent protein (n = 2) or 5 × 104 pfu of wild-type SVV (n = 2). All monkeys developed viremia and SVV-specific adaptive B- and T-cell immune responses, but none developed skin rash. At necropsy 21 days postinfection, SVV DNA was found in ganglia along the entire neuraxis and in viscera, and SVV RNA was found in ganglia, but not in viscera. The amount of SVV inoculum was associated with the extent of viremia and the immune response to virus. Our findings demonstrate that acute SVV infection of Chinese rhesus macaques leads to ganglionic infection by the hematogenous route and the induction of a virus-specific adaptive memory response in the absence of skin rash.
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